Objective To investigate the changes in expression of matrix metalloproteinases(MMPs)and the tissue inhibitor of metalloproteinases(TIMPs)from superficial venous thrombosis(SVT),varicose great saphenous veins(VGSVs),and great saphenous veins(GSVs).Methods In the venous walls of the three groups,the expression of MMP-2,MMP-9,TIMP-1,and TIMP-2 proteins,protein-positive expression ratios,mRNA expression,and protein expression were determined by immunohistochemistry,polymerase chain reaction,and Western blotting.Results The MMP-2,MMP-9,TIMP-1,and TIMP-2 protein-positive expression ratios,mRNA and protein expression in the SVT group were significantly higher than those in the VGSVs and the GSVs groups(P＜0.05).The MMP-2/TIMP-2 ratios in the SVT and the VGSVs groups were significantly lower than those in the GSVs group(P＜0.05),respectively.The MMP-2/TIMP-2 and MMP-9/TIMP-1 ratios in the SVT group were significantly lower than those in the VGSVs and the GSVs groups(P＜0.05),respectively.Conclusions Disequilibrium of MMPs and TIMPs in SVT wall occurs due to underlying high hydrostatic pressure and inflammation.These results suggested that MMPs and TIMPs participate in the process of venous wall remodeling.
关键词
浅表血栓性静脉炎/隐静脉/基质金属蛋白酶/金属蛋白酶组织抑制剂
Key words
Superficial venous thrombosis/Saphenous vein/Matrix metalloproteinases/Tissue inhibitor of metalloproteinases
维普
万方数据