Objective To investigate the function and molecular mechanism of LINC00520 in gastric cancer and to reveal the potential role of LINC00520 in gastric cancer development. Methods GEO database were screened for differentially expressed genes associated with malignant progression of gastric cancer. qPCR was performed to detect the expression of LINC00520 in gastric cancer,and cellular function experiments were performed to explore the potential effects of LINC00520. The association between LINC00520 and miR-519b-3p and HIF1A was detected by bioinformatics analysis,and their binding activities were verified by using dual luciferase reporter gene and RNA immunoprecipitation assays. Results In gastric cancer,the expression of LINC00520 was significantly increased (P<0.01),and LINC00520 promoted the malignant progression of gastric cancer cells (P<0.01). By bioinformatics,dual luciferase reporter gene and RNA immunoprecipitation assays,it was found that LINC00520 upregulated HIF1A expression by competitively targeting miR-519b-3p (P<0.01). Conclusion High expression of LINC00520 in gastric cancer plays a critical role by targeting the miR-519b-3p/HIF1A axis,which provides new targets for the diagnosis and treatment of gastric cancer.
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