创伤性脑损伤合并海水浸泡性体温过低症小鼠复温策略的初步研究
Preliminary study on the rewarming strategies in mice with traumatic brain injury combined with seawater-immersed hypothermia
叶丹 1林珑 1侯鹏伟 1张灏 2魏梁锋 2王守森2
作者信息
- 1. 福建中医药大学福总教学医院(第九○○医院)神经外科,福建福州 350025
- 2. 福建中医药大学福总教学医院(第九○○医院)神经外科,福建福州 350025;福建医科大学福总临床医学院(第九○○医院)神经外科,福建福州 350025
- 折叠
摘要
目的 通过构建小鼠创伤性脑损伤合并海水浸泡性体温过低症(TBI-SIH)模型,观察复温时程对模型小鼠损伤恢复的影响,评估电热垫复温的治疗效果.方法 48只雄性C57BL/6小鼠,随机分为4组:对照组、模型组、电热垫复温组(以小鼠核心体温升高至(37±1)℃为结束复温标志)及电热垫复温延长组(在小鼠达到正常体温后,继续使用电热垫复温1 h),每组12只.全程观察小鼠生命体征变化,并分别统计每组小鼠的死亡率.观察各组小鼠脑组织病理HE染色、尼氏染色情况,评估神经损伤程度及神经元缺失数量.24 h后行脑含水量测定及伊文思蓝染色.结果 模型组小鼠死亡率高于复温组小鼠(P＜0.05),电热垫复温组与电热垫复温延长组小鼠死亡率无明显统计学差异.每组小鼠行HE染色、尼氏染色实验后发现,小鼠脑组织病理学改变与复温密切相关.与模型组相比,复温组小鼠脑组织损伤明显改善,神经元丢失减少(P＜0.05).此外,电热垫复温组小鼠受损神经元比例少于电热垫复温延长组(P＜0.05).与对照组相比,模型组小鼠脑组织含水量及伊文思蓝渗出量明显增加,差异均有统计学意义(P＜0.05).电热垫复温后脑水肿显著减轻,伊文思蓝渗出减少(P＜0.05).然而,复温时间延长会导致伊文思蓝渗出量增加(P＜0.05).结论 复温可改善TBI-SIH小鼠脑组织损伤程度及其预后,延长复温时间对神经损伤的恢复并不明显.
Abstract
Objective By constructing a mouse model of traumatic brain injury combined with seawater-immersed hypothermia(TB1-S1H),to observe the effect of rewarming duration on the recovery of injury in model mice and to assess the therapeutic efficacy of rewarming with electric heating pads.Methods Forty-eight male C57BL/6 mice were randomly divided into 4 groups:the control group,the model group,the electric heating pad rewarming group(with the core body temperature of the mice rising to(37×1)℃ as the end of rewarming mark)and the electric heating pad rewarming extension group(after the mice reached the normal body temperature,they continued to be rewarmed for 1 h by using the electric heating pad),12 mice in each group.Vital signs of mice were observed throughout the whole process,and the mortality rate of mice in each group was counted separately.HE staining and Nissl staining were observed to assess the degree of neurological damage and the number of neuronal loss in each group,and the water content of the brain was measured and stained with Evans blue after 24 h.The mortality rate of the model group was higher than that of the rewarmed mice.Results The mortality rate of mice in the model group was higher than that of mice in the rewarming group(P＜0.05),and the mortality rate of mice in the rewarming group with electric heating pads and mice in the prolonged rewarming group with electric heating pads were comparable with no statistical difference.After HE staining and Nissl staining in each group,it was found that the histopathological changes in the brain of mice were closely related to whether or not the mice were rewarmed.Compared with the model group,the brain tissue damage of mice in the rewarming group was significantly improved,and neuronal loss was reduced(P＜0.05).In addition,the proportion of damaged neurons in the mice in the electric heating pad rewarming group was less than that in the electric heating pad rewarming prolonged group(P＜0.05).Compared with the control group,the water content of brain tissue and Evans blue exudation of mice in the model group were significantly increased,and the differences were statistically significant(P＜0.05).Cerebral oedema was significantly reduced after rewarming of the electric heating pad,and Evans blue exudation decreased(P＜0.05).However,prolonged rewarming resulted in increased Evans blue exudation(P＜0.05).Conclusion Rewarming improves the degree of brain tissue injury and its prognosis in TBI-SIH mice,and prolonged rewarming time was not significant for the recovery of neurological injury.
关键词
海水浸泡性体温过低症/颅脑损伤/小鼠/复温Key words
Seawater immersed hypothermia/Traumatic brain injury/Mice/Rewarming引用本文复制引用
基金项目
联勤医学重点专科(神经外科)项目(2022ZL01)
联勤保障部队第九○○医院战创伤救治专项指令性项目(2022ZL03)
出版年
2024
NETL
NSTL
万方数据