目的 探讨阿尔茨海默病（AD）患者脑网络功能连接（FC）变化与脑脊液（CSF）中病理标志物的相关性。 方法 选取南京大学医学院附属鼓楼医院神经内科自2020年1月至2022年12月收治的认知障碍患者39例，其中AD患者23例（AD组）、非AD患者16例（非AD组）。比较2组患者临床资料的差异；采集患者静息态功能磁共振成像（rs-fMRI）数据，采用独立分量分析2组患者脑网络间FC、网络内FC的差异，采用相关性检验分析患者脑网络间FC、网络内FC与CSF中β-淀粉样蛋白1-42（Aβ1-42）、Tau蛋白浓度之间的相关性。 结果 与非AD组相比，AD组患者CSF中Aβ1-42浓度明显较低，差异有统计学意义（P<0。05）。与非AD组比较，AD组患者视觉网络（VN）和后扣带皮层（PCC）之间、左额顶叶网络（lFPN）和前默认网络（aDMN）之间的FC明显较多，lFPN和小脑网络（CEN）之间的FC明显较少，差异均有统计学意义（P<0。05）。与非AD组比较，AD组患者的aDMN、VN和感觉运动网络（SMN）内FC均较低，lFPN内FC明显较高，差异均有统计学意义（P<0。05）。AD组患者SMN内FC和CSF中总Tau蛋白、磷酸化Tau181蛋白浓度均呈正相关关系（P<0。05），VN与PCC之间的FC和CSF中总Tau蛋白浓度呈正相关关系（P<0。05）。AD组患者aDMN、VN内FC和CSF中Aβ1-42浓度均呈正相关关系（P<0。05），FPN内FC和CSF中Aβ1-42浓度呈负相关关系（P<0。05）。 结论 AD患者大脑出现多个脑网络内部和网络之间FC的特征性变化，这些变化与CSF中Tau蛋白和Aβ1-42的浓度相关。 Objective To explore the correlations of brain network functional connectivity (FC) alterations with cerebrospinal fluid (CSF) pathological biomarkers in patients with Alzheimer's disease (AD)。 Methods A total of 39 patients with cognitive impairment， admitted to Department of Neurology， Drum Tower Hospital， Affiliated Hospital of Medical School， Nanjing University from January 2020 to December 2022 were recruited 23 patients were with AD and 16 with non-AD。 Clinical data were compared between the 2 groups。 Resting-state functional MRI (rs-fMRI) data were collected， and FC differences between brain networks and FC differences within brain networks were compared by independent component analysis。 Correlations of FC differences between brain networks and FC differences within brain networks with concentrations of β-amyloid protein 1-42 (Aβ 1-42) and Tau protein in CSF were analyzed。 Results Compared with the non-AD group， AD group had significantly lower Aβ1-42 in CSF (P<0。05)。 Compared with those in the non-AD group， FC alterations between the left frontoparietal network (lFPN) and anterior default mode network (aDMN) and between the visual network (VN) and posterior cingulate cortex (PCC)， as well as FC alterations in lFPN， were significantly increased in AD group (P<0。05)。 Compared with those in the non-AD group， FC alterations between lFPN and cerebellar network (CEN)， and FC alterations in aDMN， sensorimotor network (SMN) and VN were significantly decreased in AD group (P<0。05)。 In AD group， FC in SMN was positively correlated with total Tau and phosphorylated-Tau181 in CSF (P<0。05) FC between VN and PCC was positively correlated with total Tau in CSF (P<0。05)。 CSF Aβ1-42 was positively correlated with FC alterations in aDMN and VN， but negatively correlated with FC in FPN (P<0。05)。 Conclusion In AD patients， characteristic changes in FC within and between multiple brain networks are noted， which are related to changes of Tau protein and Aβ1-42 in CSF。
Correlations of brain network functional connectivity alterations with cerebrospinal fluid pathological markers in patients with Alzheimer's disease
Objective To explore the correlations of brain network functional connectivity (FC) alterations with cerebrospinal fluid (CSF) pathological biomarkers in patients with Alzheimer's disease (AD). Methods A total of 39 patients with cognitive impairment, admitted to Department of Neurology, Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University from January 2020 to December 2022 were recruited 23 patients were with AD and 16 with non-AD. Clinical data were compared between the 2 groups. Resting-state functional MRI (rs-fMRI) data were collected, and FC differences between brain networks and FC differences within brain networks were compared by independent component analysis. Correlations of FC differences between brain networks and FC differences within brain networks with concentrations of β-amyloid protein 1-42 (Aβ 1-42) and Tau protein in CSF were analyzed. Results Compared with the non-AD group, AD group had significantly lower Aβ1-42 in CSF (P<0.05). Compared with those in the non-AD group, FC alterations between the left frontoparietal network (lFPN) and anterior default mode network (aDMN) and between the visual network (VN) and posterior cingulate cortex (PCC), as well as FC alterations in lFPN, were significantly increased in AD group (P<0.05). Compared with those in the non-AD group, FC alterations between lFPN and cerebellar network (CEN), and FC alterations in aDMN, sensorimotor network (SMN) and VN were significantly decreased in AD group (P<0.05). In AD group, FC in SMN was positively correlated with total Tau and phosphorylated-Tau181 in CSF (P<0.05) FC between VN and PCC was positively correlated with total Tau in CSF (P<0.05). CSF Aβ1-42 was positively correlated with FC alterations in aDMN and VN, but negatively correlated with FC in FPN (P<0.05). Conclusion In AD patients, characteristic changes in FC within and between multiple brain networks are noted, which are related to changes of Tau protein and Aβ1-42 in CSF.
Alzheimer's diseaseResting-state functional magnetic resonance imagingBrain functional connectivityβ-amyloidTau protein
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