Expression of miR-34a-5p in colon cancer patients and its correlation with clinical characteristics and prognosis
Objective To investigate the expression level of miR-34a-5p in patients with colon cancer and analyze its correlation with the clinical characteristics and prognosis of these patients.Methods A prospective cohort study was conducted.Patients with colon cancer accompanied by peritoneal metastasis who underwent treatment in Xinjiang Medical University Affiliated Traditional Chinese Medicine Hospital from January 1,2021 to December 31,2021 were selected.A sample of 60 patients was planned,and the cancer focus and adjacent tissue samples of the patients were collected.Real-time quantitative PCR was used to detect the relative expression level of miR-34a-5p in the cancer focus and adjacent tissue samples,and the correlation between the relative expression level of miR-34a-5p and the clinical characteristics of colon cancer patients was analyzed.The patients with colon cancer were followed up,Kaplan-Meier curves were drawn,and prognostic factors were analyzed using COX regression.Results In the end,58 patients with colon cancer were included,and the relative expression level of miR-34a-5p in cancer focus tissues was significantly lower than that in adjacent tissues,with a statistically significant difference(P<0.001).Grouped by the average relative expression level of miR-34a-5p in cancer focus tissues of colon cancer patients,the results showed that N staging and differentiation degree were related to the expression level of miR-34a-5p.Drawing Kaplan-Meier survival curves,the results showed that the survival rate of the low expression group of miR-34a-5p in colon cancer was significantly lower than that of the high expression group,with a statistically significant difference(P=0.028).Univariate analysis showed that T staging,N staging,differentiation degree,and miR-34a-5p expression level were related to the survival period of colon cancer patients,with statistically significant differences(P<0.05).Multivariate COX regression model results suggested that differentiation degree and miR-34a-5p expression level were independent factors affecting the prognosis and survival period of colon cancer patients.Conclusion In the lesion tissues of colon cancer patients,the expression level of miR-34a-5p decreases,and the expression level of miR-34a-5p is related to N staging and differentiation degree.The expression level of miR-34a-5p is an independent factor affecting prognosis and survival period.
万方数据
维普
