Objective This study aimed to introduce the technology transfer mechanism of Mayo Clinic and explore its implications for public hospitals in China.Methods This study summarized Mayo's technology transfer concept,management mechanism,and operation model through typical case analysis.Results Mayo adhered to the core value of patient needs first and took it as the foundation for business operations.It practiced the operation management model of joint development of med-icine,education,and research,and built a sound organizational structure,high-quality talent reserves,and a complete resource support system,which assisted in the birth of high-quality and transferable achievements.Conclusions Public hospitals should optimize technology transfer from awareness,clinical orientation of basic research,translational medical system throughout the entire industry chain,specialized achievement transformation management team,and talent incentive mechanism.