Study on the Inhibitory Effects of Biochanin A on Activity of P38αMAPK
Objective To investigate the inhibitory effect of biochanin A on P38 mitogen-activated protein kinase(P38αMAPK).Methods The software Autodock Vina was used to perform molecular docking between biochanin A and the active site of P38αMAPK crystal structure 1KV2,and then the lipopolysaccharide(LPS)-induced inflammation model of mouse RAW 264.7 cells was used to verify the inhibitory effect of biochanin A on activity of P38αMAPK.Results Biochanin A could bind to the active site of 1KV2 to interfere with the entry of normal substrate of the target enzyme,which led to an in-hibitory effect on the biological functions of P38αMAPK.The experiments in cell inflammation models showed that compared with the LPS model group,after intervention with the positive control Dexamethasone,biochanin A,at monomer concentrations of 25,50 and 100 μmol/L,the levels of NO and TNF-α in cell supernatants were significantly down-regulated(P<0.05).Conclusion Biochanin A can act on the active site of P38αMAPK and exert the anti-inflammatory pharmacological activity.
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