Diagnostic Efficacy of Virulence Gene Detection and a Novel Marker for Hypervirulent Klebsiella Pneumoniae
Objective To explore the diagnostic efficacy of virulence gene detection of Klebsiella pneumoniae(KP)and novel markers for hypervirulent Klebsiella pneumoniae(HVKP).Methods According to the medical history and etiolog-ical test results,KP positive patients were selected from the hospitalized patients from 2020 to 2023,and 45 corresponding non-duplicate KP strains were obtained from the hospital bacterial bank,which were classified as the HVKP group.The KP strains of patients with bloodstream infection were randomly selected from the bacterial bank,and the strains associated with other infections were eliminated according to the specific medical history,and 48 strains were finally obtained as classic Kleb-siella pneumoniae(cKP)group.The positive rates of serotype and hypervirulent genes were compared between the two groups,and the statistically significant genetic diagnostic efficacy between the two groups was calculated.Multivariate logistic regression analysis was used to search for molecular markers for the diagnosis of HVKP.Results There was significant differ-ence in K1 type between HVKP group and cKP group(P<0.01).The top 3 virulence gene positive rates in HVKP group were PEG344,uge and fimH,respectively.Compared with cKP group,the virulence genes with significant differences were fimH,rmpA2,aero,iutA,kfuBC and PEG344(P<0.01).Diagnostic efficacy was the lowest in rmpA2,followed by fimH,aero,K1,kfuBC,iutA and PEG344.Multivariate Logistic regression analysis showed that iutA and PEG344 were influencing factors of HVKP(P<0.01).Conclusion HVKP infection is dominated by K1 serotype.rmpA2,fimH,aero,iutA,kfuBC and PEG344 genes can be used as molecular biomarkers for the diagnosis of HVKP,and PEG344 and iutA have higher diag-nostic efficacy.
万方数据
维普
