Changes in Serum miR-129-5p and miR-335-5p Levels and Clinical Significance in Hepatitis B Patients
Objective To investigate the diagnostic value of serum microRNA ( miRNA )-129-5p and miR-335-5p levels in hepatitis B patients for hepatitis B-related hepatocellular carcinoma.Methods 100 patients with hepatitis B-associated hepatocellular carcinoma ( hepatocellular carcinoma group ),50 patients with hepatitis B cirrhosis ( cirrhosis group ),and 50 patients with chronic hepatitis B ( CHB group ) admitted to our hospital from January 2021 to February 2024 were selected,and the levels of serum miR-129-5p and miR-335-5p were detected by qPCR.The relationship between serum miR-129-5p and miR-335-5p levels and hepatitis B-associated hepatocellular carcinoma was analyzed by multifactorial logistic regression;and the diagnostic value of serum AFP,miR-129-5p,and miR-335-5p levels for hepatitis B-associated hepatocellular carcinoma was analyzed by ROC curve.Results Serum miR-129-5p and miR-335-5p levels were lower in the hepatocellular carcinoma group than in the cirrhosis and CHB groups,and serum miR-129-5p and miR-335-5p levels were lower in the cirrhosis group than in the CHB group ( P<0.05 ) .After adjusting for confounders,high serum miR-129-5p and miR-335-5p levels were independent protective factors for hepatitis B-associated hepatocellular carcinoma ( P<0.05 ) .The area under the curve for the combined diagnosis of hepatitis B-associated hepatocellular carcinoma by serum alpha-fetoprotein ( AFP ),miR-129-5p,and miR-335-5p levels was 0.984,which was greater than that for the diagnosis of AFP,miR-129-5p,miR-335-5p,miR-129-5p+miR-335-5p of 0.812,0.814,0.820,and 0.876 ( P<0.05 ) .Conclusion Reduced levels of serum miR-129-5p and miR-335-5p in patients with hepatitis B were independently associated with hepatitis B-associated hepatocellular carcinoma,and serum miR-129-5p and miR-335-5p in combination with AFP were of high value in diagnosing hepatitis B-associated hepatocellular carcinoma.
Hepatitis BHepatic tumorMicroRNA-129-5pMicroRNA-335-5pROC curveArea under the curveLogistic model
万方数据
维普
