Exploring the Impact of Minimally Invasive Puncture Therapy Assisted by 3D Printing Technology on Patients with Hypertensive Cerebral Hemorrhage Based on Serum Metabolomics
Objective To explore the impact of minimally invasive puncture therapy assisted by 3D printing tech-nology on patients with hypertensive cerebral hemorrhage and analyze the related metabolic pathways using the ultra-per-formance liquid chromatography-mass spectrometry (UPLC-MS) combined method. Methods This study selected 128 patients with hypertensive cerebral hemorrhage who were treated in Lincheng County People's Hospital from January 2022 to August 2023. They were randomly divided into an experimental group (64 cases,receiving minimally invasive hemato-ma removal assisted by 3D printing technology) and a control group (64 cases,receiving traditional craniotomy for hema-toma evacuation). The intraoperative blood loss,operation time,hematoma clearance rate,pre-and post-operative NIHSS scores of the two groups were compared,and the serum levels of TNF-α,IL-6,CRP,and brain-derived neurotrophic factor (BDNF) on the 7th day after the operation were recorded. Serum samples were collected for targeted metabolomics analysis. Results Compared with the control group,the experimental group had less intraoperative blood loss,shorter operation time,and better hematoma clearance effect (P<0.05). The serum BDNF level increased on the 7th day after the operation,while the levels of inflammatory biomarkers (TNF-α,IL-6,CRP) decreased (P<0.05). Non-targeted metabolomics analysis showed that there were significant differences in the metabolic levels between the experimental group and the control group (P<0.05). Differential metabolite analysis identified 110 significantly changed metabolites involving multiple categories. KEGG en-richment analysis indicated that these metabolites play critical roles in biological metabolism. Conclusions Minimally invasive puncture therapy assisted by 3D printing technology showed better metabolic regulation effects in patients with hypertensive intracerebral hemorrhage. The changes of metabolites and enriched metabolic pathways provide new perspectives for improving treat-ment strategies and researching disease mechanisms.
万方数据
维普
