The Expression Level and Significance of IL-17,IL-10 and Foxp3 in Peripheral Blood Mononuclear Cells of Children with Mycoplasma Pneumoniae
Objective To investigate the expression and clinical significance of interleukin (IL-17),IL-10,fork-head transcription factor P3 (Foxp3) mRNA levels in peripheral blood mononuclear cells (PBMC) of children with myco-plasma pneumoniae (MPP). Methods A total of 110 MPP cases admitted from January 2023 to May 2024 were divided into a mild group (75 cases) and a severe group (35 cases) according to MPP severity,and 50 healthy children in the same period were selected as the control group. PBMC was isolated by blood collection,and the expression of IL-17,IL-10,and Foxp3 mRNA levels in PBMC of each group was measured and compared. The diagnostic efficacy of each index for severe MPP was analyzed by ROC curve. Results In terms of the expression of PBMC IL-17 mRNA level:severe group>mild group>control group (P<0.05);in terms of the expression of IL-10 and Foxp3 mRNA levels:severe group<mild group<control group (P<0.05). Before treatment,The areas under the curve (AUC) of PBMC IL-17,IL-10,and Foxp3 mRNA ex-pression levels for diagnosing severe MMP were 0.953,0.913,and 0.908,respectively. The severity of MMP was positively correlated with the level of PBMC IL-17 mRNA expression (P<0.05) and negatively correlated with the levels of IL-10 and Foxp3 mRNA expression (P<0.05). Conclusions The upregulated expression of PBMC IL-17 mRNA and downregulat-ed expression of IL-10 and Foxp3 mRNA in children with MPP might be related to MPP severity. Detection of the expression levels of PBMC IL-17,IL-10,and Foxp3 mRNA has high diagnostic value for severe MPP.
Mycoplasma pneumoniaeChildrenPeripheral blood mononuclear cellsInterleukin-17Interleukin-10Forkhead transcription factor P3Receiver Operating Characteristic curveArea Under the Curve (AUC)
万方数据
维普
