首页|胫骨骨折患者血清miR-378、ADAM10水平与术后延迟愈合的关系及预测能效

胫骨骨折患者血清miR-378、ADAM10水平与术后延迟愈合的关系及预测能效

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目的 探讨胫骨骨折患者血清微小RNA-378(miR-378)、解整合素金属蛋白酶10(ADAM10)水平与术后延迟愈合的关系及预测能效.方法 选取2020年6月至2023年12月在山东省青岛市黄岛区中医医院行内固定术的胫骨骨折患者150例,根据内固定术后是否延迟愈合分为延迟愈合组(n=42)和正常愈合组(n=108),采用实时荧光定量PCR和酶联免疫吸附法检测血清miR-378、ADAM10水平.以胫骨骨折患者术后延迟愈合为因变量,多因素非条件Logistic回归确定其影响因素,绘制受试者工作特征(ROC)曲线分析血清miR-378、ADAM10水平对其的预测能效.结果 150例胫骨骨折患者术后延迟愈合发生率为28.00％(42/150).延迟愈合组血清miR-378、ADAM10水平(1.04±0.17)、(1572.70±562.06)pg/mL高于正常愈合组(0.87±0.13)、(1050.23±291.67)pg/mL(t=5.984、5.732,P均<0.05).胫骨骨折患者术后延迟愈合的独立危险因素为年龄(OR=1.053,95％CI:1.005～1.103,P=0.029)、miR-378(OR=2.362,95％CI:1.596～3.495,P<0.001)、ADAM10(OR=1.003,95％CI:1.002～1.005,P<0.001).血清miR-378、ADAM10水平联合预测胫骨骨折患者术后延迟愈合的曲线下面积(AUC)为0.881(95％CI:0.818～0.928),大于血清miR-378、ADAM10水平单独预测的0.789(95％CI:0.715～0.851)、0.788(95％CI:0.714～0.851)(Z=2.912、2.686,P均<0.05).结论 胫骨骨折患者血清miR-378、ADAM10水平升高与术后延迟愈合密切相关,血清miR-378、ADAM10水平联合对其有较高的预测能效.
Relationship Between Serum Mir-378 and Adam10 Levels and Postoperative Delayed Union in Patients with Tibial Fractures and Predictive Energy Efficiency
Objective To investigate the relationship between serum microRNA-378 (miR-378) and a disintegrin and metalloprotease 10 (ADAM10) levels and postoperative delayed union in patients with tibial fractures and their predictive value. Methods A total of 150 tibial fracture patients who underwent internal fixation from June 2020 to December 2023 were se-lected and divided into the delayed union group (42 cases) and the normal healing group (108 cases) based on whether delayed union occurred after internal fixation. Serum miR-378 and ADAM10 levels were detected by real-time fluorescence quantita-tive PCR and enzyme-linked immunosorbent assay. Using delayed union after surgery in tibial fracture patients as the depen-dent variable,multivariate unconditional Logistic regression was employed to determine influencing factors,and the receiver operating characteristic (ROC) curve was plotted to analyze the predictive efficacy of serum miR-378 and ADAM10 levels. Results The incidence of postoperative delayed union in 150 tibial fracture patients was 28.00％ (42/150). Serum miR-378 (1.04±0.17) and ADAM10 (1572.70±562.06) pg/mL levels in the delayed union group were significantly higher than those in the normal healing group (0.87±0.13) and (1050.23±291.67) pg/mL (t=5.984,5.732,all P<0.05). Independent risk factors for postoperative delayed union in tibial fracture pa-tients included age (OR=1.053,95％CI:1.005 to 1.103,P=0.029),miR-378 (OR=2.362,95％CI:1.596 to 3.495,P<0.001),and ADAM10 (OR=1.003,95％CI:1.002 to 1.005,P<0.001). The area under the ROC curve for the combination of serum miR-378 and ADAM10 lev-els in predicting postoperative delayed union was 0.881 (95％CI:0.818 to 0.928),which was significantly greater than that for miR-378 (0.789,95％CI:0.715 to 0.851) or ADAM10 (0.788,95％CI:0.714 to 0.851) alone (Z=2.912,2.686,all P<0.05). Conclusion Elevated serum miR-378 and ADAM10 levels are closely associated with delayed union after tibial fracture surgery. The combination of these markers demonstrates high predictive efficacy.

Tibial fractureMicroRNA-378A disintegrin and metalloprotease 10Postoperative delayed unionPredictive efficacy

杨震、李慧、宋龙强

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266500 山东 青岛,山东省青岛市黄岛区中医医院:骨伤二科

胫骨骨折 微小RNA-378 解整合素金属蛋白酶10 术后延迟愈合 预测能效

2024

10.3639/j.issn.2095-3097.2024.05.023

转化医学杂志
海军总医院

转化医学杂志

CSTPCD
影响因子：0.671
ISSN：2095-3097
年,卷(期)：2024.13(5)