The effect of sleep deprivation on the hair growth cycle of mice
赵钧 1徐伟力 2周玥 3竺璐 1周易 1张菊芳 4丁国兰 孙恒赟
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作者信息
1. 浙江省皮肤病医院皮肤二科，湖州 313000
2. 西湖大学医学院附属杭州市第一人民医院城北院区烧伤整形科，杭州 310000
3. 解放军联勤保障部队第九○三医院烧伤整形科，杭州 310000
4. 西湖大学医学院附属杭州市第一人民医院医疗美容科，杭州 310000
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摘要
目的 探讨睡眠剥夺对小鼠毛发生长周期的影响。 方法 将72只成年C57BL/6J小鼠采用抽签法随机分为对照组和实验组，每组36只。对照组小鼠脱毛后在水平台环境下常规饲养，每天16 h；实验组小鼠脱毛后通过改良水平台的方法进行睡眠剥夺，每天16 h。于实验后第1、9、19天，取2组小鼠脱毛区皮肤组织制作HE染色切片，通过光学显微镜观察毛囊形态，对小鼠毛发进行分期和评分：以休止期为起始点，设为0分；生长期Ⅰ～Ⅴ期分别设为1～5分；生长期Ⅵ期及退行期Ⅰ期设为6分；退行期Ⅱ～Ⅷ期分别设为7～13分。采用SPSS 26.0软件进行统计学分析，毛发周期评分以±s表示，组间同一时间点评分比较采用独立样本t检验，组内不同时间点评分比较采用韦尔奇检验，P<0.05为差异有统计学意义。 结果 第1、9、19天，对照组小鼠毛发周期评分分别为(1.00±0.57)、(5.04±0.94)、(9.52±0.87)分，实验组分别为(0.85±0.62)、(2.40±0.50)、(6.08±0.42)分。组间比较显示，3个时间点实验组毛发周期评分均低于对照组，第1天差异无统计学意义(t＝1.03，P＝0.307)，第9天(t＝13.38，P<0.001)及第19天(t＝16.41，P<0.001)差异均有统计学意义；组内比较显示，对照组和实验组3个时间点间毛发周期评分比较差异均有统计学意义(P均<0.01)，评分随时间推移而增加。 结论 睡眠剥夺会造成小鼠毛发生长周期循环的滞后，但不会造成毛发生长周期循环停止，滞后的程度随着剥夺天数的增加而逐渐增大。 Objective To explore the effect of sleep deprivation on hair growth cycle in mice. Methods Seventy-two adult C57BL/6J mice were randomly divided into a control group of 36 mice and an experimental group of 36 mice using a lottery method. After hair removal, in the control the group the mice were subjected to routine feeding in a horizontal platform environment for 16 hours per day, while in the experimental group the mice were subjected to sleep deprivation using an improved water platform for 16 hours per day. On the 1st, 9th, and 19th day after the experiment, the skin tissue from the hair removal area of the two groups of mice was taken for HE staining. Then the mouse hair was staged and scored by observing hair follicle morphology under an optical microscope. The cycle and score of the hair was started from the telogen, which was set as 0 points the anagen Ⅰ to V were set as 1-5 points the anagen Ⅵ and catagen Ⅰ were set as 6 points the catagen Ⅱ to Ⅷ were set at 7-13 points. Statistical analysis was conducted by SPSS 26.0. The hair cycle score was expressed as Mean±SD. Student’s t-test was used for comparison between groups at the same time point, and Welch-test was used for comparison of scores at different time points within the group. P<0.05 indicated a statistically significant difference. Results On the 1st, 9th, and 19th day, the hair cycle scores of the control group were 1.00 ± 0.57, 5.04 ± 0.94, and 9.52 ± 0.87 points, while the hair cycle scores of the experimental group were 0.85 ± 0.62, 2.40 ± 0.50 and 6.08 ± 0.42 points. Inter group comparison showed that the hair cycle scores of the experimental group were lower than those of the control group at all three time points. There was no statistically significant difference on the 1st day (t=1.03, P=0.307), while the differences were statistically significant on the 9th day (t=13.38, P<0.001) and the 19th day (t=16.41, P<0.001). Intragroup comparison showed that there was a statistically significant difference in hair cycle scores between the control group and the experimental group at different time points(P<0.01), and the scores increased over time. Conclusion Sleep deprivation can cause a lag in the hair growth cycle of mice, but it does not cause a cessation of the hair growth cycle. The degree of lag gradually increases with the number of deprivation days.
Abstract
Objective To explore the effect of sleep deprivation on hair growth cycle in mice. Methods Seventy-two adult C57BL/6J mice were randomly divided into a control group of 36 mice and an experimental group of 36 mice using a lottery method. After hair removal, in the control the group the mice were subjected to routine feeding in a horizontal platform environment for 16 hours per day, while in the experimental group the mice were subjected to sleep deprivation using an improved water platform for 16 hours per day. On the 1st, 9th, and 19th day after the experiment, the skin tissue from the hair removal area of the two groups of mice was taken for HE staining. Then the mouse hair was staged and scored by observing hair follicle morphology under an optical microscope. The cycle and score of the hair was started from the telogen, which was set as 0 points the anagen Ⅰ to V were set as 1-5 points the anagen Ⅵ and catagen Ⅰ were set as 6 points the catagen Ⅱ to Ⅷ were set at 7-13 points. Statistical analysis was conducted by SPSS 26.0. The hair cycle score was expressed as Mean±SD. Student’s t-test was used for comparison between groups at the same time point, and Welch-test was used for comparison of scores at different time points within the group. P<0.05 indicated a statistically significant difference. Results On the 1st, 9th, and 19th day, the hair cycle scores of the control group were 1.00 ± 0.57, 5.04 ± 0.94, and 9.52 ± 0.87 points, while the hair cycle scores of the experimental group were 0.85 ± 0.62, 2.40 ± 0.50 and 6.08 ± 0.42 points. Inter group comparison showed that the hair cycle scores of the experimental group were lower than those of the control group at all three time points. There was no statistically significant difference on the 1st day (t=1.03, P=0.307), while the differences were statistically significant on the 9th day (t=13.38, P<0.001) and the 19th day (t=16.41, P<0.001). Intragroup comparison showed that there was a statistically significant difference in hair cycle scores between the control group and the experimental group at different time points(P<0.01), and the scores increased over time. Conclusion Sleep deprivation can cause a lag in the hair growth cycle of mice, but it does not cause a cessation of the hair growth cycle. The degree of lag gradually increases with the number of deprivation days.
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