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白菜和芜菁杂种小孢子培养研究

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对白菜和芜菁的6个杂种F1代进行游离小孢子培养,结果表明,不同组合间小孢子胚胎发生频率存在很大差异,胚产量最高的两个组合“矮脚黄×气死孩”和“苏州青×气死孩”平均每蕾分别产生21.1和17.2个胚。诱导杂种胚状体发生的最佳时期是单核中期至单核靠边期。33℃、24h热激处理可明显提高杂种小孢子胚的诱导率,培养基中加入活性炭也可改善胚的分化。
Study on microspore culture of the hybrids between Chinese cabbage and turnip
Microspores of six hybrids between non-headed Chinese cabbage(Brassica campestris ssp. chinensis) and turnip (B. campestris ssp. rapifera Matzg) were cultured . The embryoid yield varied with the different materials used, and the two genotypes with the highest embryoid yield, B. campestris ssp. chinensis cv. aijiaohuang and shuzhouqing crossed with B. campestris ssp. rapifera Matzg cv. qisihai produced 21.2 and 17.2 embryoids per flower bud. The optimum period of inducing embryo was uninucleate period. Preheat of 33 ℃ for 24 h before the microspores were transferred to the culture condition of 25 ℃ could obviously enhance the yields, and the active carbon could improve embryo quality.

卢钢、曹家树

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浙江大学蔬菜研究所,浙江杭州310029

白菜 芜菁 杂种 小孢子培养

国家自然科学基金

39870509

2001

浙江大学学报(农业与生命科学版)
浙江大学

浙江大学学报(农业与生命科学版)

CSCD
影响因子:0.725
ISSN:1008-9209
年,卷(期):2001.27(2)
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