Identification of SRAP molecular markers linked to powdery mildew resistance in cucumber
With a F2 population between a resistant parent and susceptible parent, SRAP analysis was carried to develop molecular markers linked to powdery mildew in cucumber. Sixty-two pairs of primer combinations which had stable amplified polymorphic bands between resistant parent and susceptible parent were selected from 437 pairs of SRAP primer combinations. Further analyzing the F2 segregating populations using these primers, one SRAP marker of Mel/Em9--284 bp linked to the powdery mildew resistance was found, with 9.8 cM genetic distance. The marker can be used for cloning disease-resistant gene and advancing efficiency of disease-resistant breeding on molecular marker assisted selection.
NETL
NSTL
万方数据
维普
