Effects of different growth regulators on germination and main carbon-nitrogen metabolites contents during the storage period of potato tuber
The regulation of dormancy is very essential in the production of potato.To satisfy the demand of different sowing dates and to keep high rate of emergence and growth potential in seed potato, it always requires prolonging or shortening the dormancy stage to guarantee the field emergence.Meanwhile, when potatoes are treated as food, the dormancy period should be prolonged as long as possible to keep the potatoes fresh.Physical and plant growth regulator treatments are two main methods that regulate the dormancy in potato.The physical treatment requires high cost of labor and material resources, while the plant growth regulator treatment has advantages of convenience and cost saving over the physical treatment.The conventional plant growth regulators were used to adjust the plant dormancy including sprouting inhibitors chlorpropham (CIPC), abscisic acid (ABA), and sprouting promoter gibberellin A3 (GA3).There were numerous reports about the regulating effects of these regulators, but only a few investigations have been done to compare the regulating effects of these regulators on potato.To comprehensively study the effects of different growth regulators on the germination and main carbonnitrogen metabolites contents of potato during storage, a laboratory experiment was conducted from May to August in 2014.The potato cultivar "Chuanyu-117" was used in this research.Potato tubers were dipped in aqueous solutions of CIPC, ABA and GA3 with efficacious concentrations for 30 minutes, respectively.The results indicated that GA3 could release potato dormancy in advance, and the intensity and amplitude of tubers dormancy, when treated with GA3, reduced by 17 days and 11 days, respectively, the length/diameter ratio of bud was much higher than that of control (CK), showing that GA3 could accelerate potato tubers germination and bud growth.Whereas, ABA significantly prolonged the dormancy, the dormancy amplitude reduced by 11 days but the dormancy intensity increased by 6 days, and the length/diameter ratio of bud was lower than that of CK, which showed that ABA could accelerate the speed, vigor and uniformity of bud growth after germination.The CIPC had a more obviously suppressive effect with a 70-day interval between the beginning of treatment and germination, and the germination period was also expanded to at least 40 days in contrast with CK.The change regulations of carbon-nitrogen metabolites contents in different treatments were the same, but the degrees of changes were quite different.The starch contents of potatoes treated with GA3, ABA and CIPC for 12 weeks reduced by 13.36％, 11.30％ and 5.93％, respectively;while the soluble sugar contents reduced by 48.3％, 58.9％ and 56.1％, respectively, when compared with those of the beginning of treatments.Both of the soluble protein content and the crude protein content showed a high-low trend line.The soluble protein content of GA3, ABA and CIPC treatment at the previous time of storage increased by 25.73％, 39.68％ and 31.32％, respectively, and then reduced by 19.17％, 33.22％ and 17.74％ at later storage period, respectively.The crude protein content of GA3, ABA, and CIPC treatments increased by 4.77％, 12.67％ and 12.65％ at the previous time of storage, respectively, and then reduced by 11.37％, 18.02％ and 8.71％ at later storage period, respectively.The C/N ratio of the sprouting promoter treatment was higher than those of the sprouting inhibitor treatments, and all of them showed a downward tendency.In conclusion, GA3 could release the dormancy of potato in advance, the bud grew faster and the carbonnitrogen metabolism activity was strong.Whereas, CIPC could prolong the dormancy period and inhibit the bud growth, the carbon-nitrogen metabolism activity of potato tuber was weak and the nutrient consumption was also less.ABA also prolonged the dormancy period but enhanced the germination uniformity and bud growth and the carbon-nitrogen metabolism activity after germination was strong.
NETL
NSTL
万方数据
维普
