Study on influencing factors of bulblet enlargement in vitro of Lilium amoenum
Lilium amoenum is a rare lily germplasm with an agreeable rose fragrance.This study aimed to explore the optimal culture conditions and medium formula for the enlargement of L.amoenum bulblets in vitro.Bulblets of L.amoenum with a diameter of 0.4-0.6 cm were inoculated on Murashige&Skoog(MS)medium supplemented with different concentrations of sucrose or sucrose combined with naphthalene acetic acid(NAA),N-(phenylmethyl)-9H-purin-6-amine(6-BA),indole butyric acid(IBA),or paclobutrazol(PP333).The culture conditions of 16 hours of light/8 hours of darkness and full darkness were set up.After 45 days of culture,the enlargement coefficient and diameter increase multiple of the bulblets were counted.The results showed that in the range of experimental concentrations,with the increase of sucrose and hormone concentrations,the enlargement coefficient and diameter increase multiples of L.amoenum bulblets overall first increased and then decreased.When the sucrose concentration was 50-120 g/L,the bulblet enlargement effect at 90 g/L sucrose was the greatest,and the bulblets were green and shiny,and had good cohesion.When 50-120 g/L sucrose was combined with 0.1-0.7 mg/L NAA/6-BA,0.5-4.0 mg/L IBA or 5-40 mg/L PP333,the effect of bulblet enlargement was the greatest at 70 g/L sucrose and 1.0 mg/L IBA,and the enlargement coefficient and diameter increase multiple were 3.63 and 1.83,respectively.The synergistic effect of high concentrations of sucrose and PP333 significantly inhibited the enlargement of bulblets.Lilium amoenum bulblets cultured under 16 hours of light/8 hours of darkness grew better than those cultured under full darkness,but the rooting effect was greater under full darkness.This study provides a scientific basis for shortening bulb growth time in the field and for germplasm resource protection.
Lilium amoenumbulbletsenlargement in vitrorapid propagation system
NETL
NSTL
万方数据
维普
