Composition and dynamic change characteristics of human pathogenic communities in dryland farmland with manure application
To understand the community structure composition and dynamic change characteristics of human pathogenic bacteria(HPB)in soils after manure application,laboratory cultivation experiments were conducted on agricultural soils with long-term application of chicken manure,pig manure,or chemical fertilizer in five regions of Hangzhou,Jiaxing,Quzhou,Jinhua,and Longyou in Zhejiang Province,and the community compositions of the soil bacteria and HPB were analyzed by high-throughput sequencing and sequence alignment methods.The results showed that a total of 75 HPB were detected in 160 soil samples and two manure samples,and the dominant HPB were Bacillus_megaterium_QM_B1551(24.2%)and Clostridium_beijerinckii_NCIMB_8052(23.1%).The Shannon indexes of bacteria and HPB in the soils decreased after the application of pig manure,while the diversities of bacteria and HPB in the soils with the application of chicken manure or chemical fertilizer had no significant changes.The results of the principal coordinate analysis showed that there was a significant difference in the bacterial community composition of soils between the manure treatment and the unfertilized control,especially in the pig manure treatment(P<0.001);22.7%of all HPB were shared among the soil samples;and the relative abundance of most HPB in the soils treated with manure was higher than that in the unfertilized control,and it decreased continuously with the extension of cultivation time.The results of the variance partitioning analysis showed that soil physicochemical properties,bacterial communities,and their interactions were important factors contributing to the variation of HPB in the soils.In summary,the HPB variation characteristics in soils treated with manure are influenced mainly by manure types,soil types,soil physicochemical properties,and inherent bacterial communities.
human pathogenic bacteriamanurebacterial communitysoildynamic change
NETL
NSTL
万方数据
维普
