Characteristics of microbial communities and their association with immunity and metabolism at the early developmental stage of Protosalanx chinensis
In order to understand the changes and characteristics of microbial communities during early development of Protosalanx chinensis,samples were collected at five developmental periods,which are the embryonic period(heartbeat stage,XT),the endogenous nutrition period(the first day after hatching,H1),the mixed nutrition period(the fourth day after hatching,H4),the open feeding period(the seventh day after hatching,H7),and the exogenous nutrition period(the tenth day after hatching,H10).Microbial community succession during early development was observed by 16S rRNA gene sequencing technology,especially the characteristics and key microbial genera before and after feeding.Combined with the transcriptome data of the same batch of samples,the microbial genera related to immunity and metabolism were analyzed based on the association network method.The results showed that there were significant differences in β diversity among the different periods at the early developmental stage(P<0.001).The dominant bacteria in the XT period were Flavobacterium and Chryseobacterium,and the dominant bacterium in the H1 period was Pseudomonas.The main bacteria in the H4 period were Flavobacterium and Pseudomonas.The main bacteria in H7 and H10 periods were Flectobacillus and Pseudomonas.The abundance of Pseudomonas was stable at all developmental periods.Node bacteria such as Flectobacillus were significantly associated with the expression of various immune and metabolic genes.This study has obtained the microbial succession information of the early developmental stage of P.chinensis for the first time,and screened out the dominant bacteria and node bacteria,which will provide references and ideas for the scaled cultivation of P.chinensis fry.
NETL
NSTL
万方数据
维普
