烟草BTB/POZ蛋白家族的鉴定及其在抗马铃薯Y病毒中的作用
Identification of BTB/POZ protein family in Nicotiana tabacum and its role in resistance to potato virus Y(PVY)
张劭文 1赵天伦 2肖钦之 3祝水金 2陈进红2
作者信息
- 1. 浙江大学农业与生物技术学院,浙江 杭州 310058
- 2. 浙江大学农业与生物技术学院,浙江 杭州 310058;浙江大学海南研究院,海南 三亚 572025;浙江省作物种质资源重点实验室,浙江杭州 310058
- 3. 湖南省烟草公司永州市公司,湖南 永州 425000
- 折叠
摘要
马铃薯Y病毒(potato virus Y,PVY)侵染烟草(Nicotiana tabacum)会引起烟草脉带病,造成烟叶品质下降.broad complex,tramtrack,and bric-à-brac/pox virus and zinc finger(BTB/POZ)是动植物体内广泛存在的蛋白质家族,在植物生长和发育的各个环节都起着非常重要的作用.本研究鉴定到90个烟草BTB/POZ家族蛋白,对应71个基因.在这些蛋白质的序列中,识别到9种保守基序(motif),并根据其出现顺序将BTB/POZ家族成员划分至6个亚家族.结构域分析表明,BTB/POZ同一亚家族成员的结构域具有一致性.对BTB/POZ蛋白的三维构象进行预测发现,所有亚家族成员结构都以α螺旋为主,同一亚家族成员的三维构象相似.烟草品种K326和突变体M867(抗PVY)的BTB/POZ家族基因表达模式分析显示,叶片接种PVY后,NtBTB2M、NtBTB2K、NtBTB2L、NtBTB6E基因的表达丰度显著增加,这可能与烟草M867对PVY的抗性较强有关.顺式作用元件分析显示,NtBTB2K、NtBTB2L、NtBTB2M和NtBTB6E基因的上游2 000 bp区域内含有若干逆境响应相关元件,包括水杨酸响应元件、MYB结合位点等,可能和这些基因的上调表达有关.本研究为BTB/POZ蛋白的功能研究提供了理论依据,为烟草抗病育种提供了一定参考.
Abstract
The infection of Nicotiana tabacum with potato virus Y(PVY)can cause tobacco vein disease,leading to a decrease in the tobacco leaf quality.The broad complex,tramtrack,and bric-à-brac/pox virus and zinc finger(BTB/POZ)family exists widely in plants and animals.It plays a very important role in various stages of plant growth and development.In this study,90 proteins in the BTB/POZ family of N.tabacum were identified,corresponding to 71 genes.Among these protein sequences,nine conserved motifs were identified,and the members of the BTB/POZ family were divided into six subfamilies according to the order of their appearance.Domain analysis showed that the domains of members of the same subfamily in BTB/POZ are consistent.The three-dimensional(3D)conformation of the BTB/POZ protein was predicted based on the amino acid sequence.All the members of the subfamily are mainly structured with α helixes,and the 3D conformations of members of the same subfamily are similar.Analysis of the BTB/POZ family gene expression patterns of N.tabacum K326 and mutant M867(anti-PVY)showed that the expression levels of NtBTB2K,NtBTB2L,NtBTB2M and NtBTB6E increased significantly after inoculation with PVY,which may be related to the strong resistance of N.tabacum M867 to PVY.Cis-acting element analysis showed that the 2 000 bp upstream region of NtBTB2K,NtBTB2L,NtBTB2M and NtBTB6E contained several stress responsive elements,including TCA-element and MYB-binding site,which may be related to the upregulated expression of these genes.This study provides a theoretical basis for the study of BTB/POZ protein function,and provides a reference for disease resistance of tobacco breeding.
关键词
烟草/BTB/POZ家族/保守基序/顺式作用元件Key words
Nicotiana tabacum/BTB/POZ family/conserved motif/cis-acting element引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据