Identification of BTB/POZ protein family in Nicotiana tabacum and its role in resistance to potato virus Y(PVY)
The infection of Nicotiana tabacum with potato virus Y(PVY)can cause tobacco vein disease,leading to a decrease in the tobacco leaf quality.The broad complex,tramtrack,and bric-à-brac/pox virus and zinc finger(BTB/POZ)family exists widely in plants and animals.It plays a very important role in various stages of plant growth and development.In this study,90 proteins in the BTB/POZ family of N.tabacum were identified,corresponding to 71 genes.Among these protein sequences,nine conserved motifs were identified,and the members of the BTB/POZ family were divided into six subfamilies according to the order of their appearance.Domain analysis showed that the domains of members of the same subfamily in BTB/POZ are consistent.The three-dimensional(3D)conformation of the BTB/POZ protein was predicted based on the amino acid sequence.All the members of the subfamily are mainly structured with α helixes,and the 3D conformations of members of the same subfamily are similar.Analysis of the BTB/POZ family gene expression patterns of N.tabacum K326 and mutant M867(anti-PVY)showed that the expression levels of NtBTB2K,NtBTB2L,NtBTB2M and NtBTB6E increased significantly after inoculation with PVY,which may be related to the strong resistance of N.tabacum M867 to PVY.Cis-acting element analysis showed that the 2 000 bp upstream region of NtBTB2K,NtBTB2L,NtBTB2M and NtBTB6E contained several stress responsive elements,including TCA-element and MYB-binding site,which may be related to the upregulated expression of these genes.This study provides a theoretical basis for the study of BTB/POZ protein function,and provides a reference for disease resistance of tobacco breeding.
Nicotiana tabacumBTB/POZ familyconserved motifcis-acting element
NETL
NSTL
万方数据
