在生物被膜中活但不可培养(viable but nonculturable,VBNC)状态细胞的形成与调控是微生物学研究的前沿课题.尽管过去对VBNC状态的概念存在争议,但其对生态平衡和公共安全产生的重要影响不容忽视.多因素调控VBNC状态细胞的形成已逐渐被揭示,包括基因表达和蛋白质合成等途径.这些研究奠定了解析VBNC状态细胞生存策略和生态适应性的基础.本综述从多角度对VBNC状态细胞进行了总结,包括其定义的讨论,生物被膜内VBNC状态细胞存在情况、形成的诱因及相关机制,VBNC状态细胞的调控及防控等.未来应聚焦于探索VBNC状态细胞的功能及意义,以创新方式干预VBNC状态形成,深入揭示VBNC状态细胞的分子机制和代谢途径.
Research progress on the formation and regulation of viable but nonculturable cells in biofilms
The formation and regulation of viable but nonculturable(VBNC)cells in biofilms is a cutting-edge topic in microbiology research.Although there has been controversy over the concept of the VBNC state in the past,its significant impact on ecological balance and public safety cannot be denied.The formation of VBNC cells regulated by multiple factors has gradually been revealed,including pathways such as gene expression and protein synthesis.These studies lay the foundation for analyzing the survival strategies and ecological adaptability of VBNC cells.This review summarized VBNC cells from multiple perspectives,including the discussion of their definition,the presence of VBNC cells in biofilms,their formation triggers and relevant mechanisms,and the regulation and prevention of VBNC cells.The focus of future research will be to explore the function and significance of VBNC cells,intervene in their formation,solve related problems in innovative ways,and reveal the molecular mechanisms and metabolic pathways of VBNC cells in detail.
viable but nonculturable cellsbiofilmregulatory mechanism
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