Effects of Huoshanhuangya tea extract on lipid accumulation and inflamma-tory response in palmitic acid-induced cells
This study aimed to compare and analyze the major biochemical components and antioxidant activities of four different grades(special-grade 1,special-grade 2,grade 1 and grade 2)of Huoshanhuangya tea.Special-grade 1 Huoshanhuangya tea was chosen to study the effects of Huoshanhuangya tea extract(HTE)on lipid accumulation and proinflammatory cytokines via palmitic acid-induced alpha mouse liver 12(AML-12),BV-2,human kidney-2(HK-2),and human gastric adenocarcinoma(AGS)cells.The results revealed that the special-grade 1 Huoshanhuangya tea had the lowest soluble sugar content,the highest caffeine content,catechins content and total free amino acid content,and the strongest antioxidant activity.After treatment with HTE at a certain concentration,the viability of palmitic acid-induced AML-12,BV-2,HK-2 and AGS cells significantly increased;the lipid accumulation induced by palmitic acid was inhibited;and the release levels of proinflammatory cytokines such as tumor necrosis factor-α(TNF-α)in palmitic acid-induced AML-12,BV-2 and HK-2 cells were significantly decreased.To further explore its mechanism of action,it was found that HTE down-regulated the expression of Toll-like receptor 4(TLR4)and nuclear factor-κB RelA(NF-κB p65)in the inflammatory signaling pathway of BV-2 cells,suggesting that HTE may relieve the palmitic acid-induced inflammatory damage of BV-2 cells by inhibiting the activation of the TLR4/NF-κB signaling pathway.
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