为探究NUDIX(nucleoside diphosphates linked to other moieties X)基因家族在月月粉月季(Rosa chinensis'Old Blush')中的功能及潜在应用，本研究利用生物信息学方法，共鉴定出41个月月粉月季NUDIX基因家族成员，并对其系统发育关系、基因结构和表达模式等进行了分析。构建月月粉月季与野蔷薇、玫瑰、光叶蔷薇、草莓、苹果、拟南芥、水稻的NUDIX蛋白系统发育树，结果发现，NUDIX蛋白可分成13个亚家族，其中NUDIX1、NUDIX2、NUDIX4、NUDIX11亚家族成员数量较多。基因共线性分析发现，月月粉月季NUDIX基因发生了全基因组复制事件，并产生2对共线性基因对。染色体定位结果显示，月月粉月季NUDIX基因发生多次串联重复事件，这些重复事件大多经历了纯化选择。转录组数据分析表明，RcNUDIX1a/1b/1d/1e在花瓣组织中特异性表达，RcNUDIX1b/1e/4c/9/11d可能参与叶片的胁迫响应，RcIDI可能参与植物组织幼根的发育和早期花发育，RcNUDIX2b/2d/2h可能参与花器官的衰老调控。综上所述，月月粉月季NUDIX基因的数量扩增主要由串联重复事件引起，NUDIX基因家族可能对月月粉月季次生代谢、生长发育和叶片胁迫响应等生理过程发挥着重要的调控作用。本研究为进一步探究月月粉月季NUDIX基因功能提供了理论依据。
Identification and expression analysis of NUDIX gene family in Rosa chinensis 'Old Blush'
To explore the function and potential application of NUDIX (nucleoside diphosphates linked to other moieties X) gene family in Rosa chinensis 'Old Blush',41 NUDIX gene family members of Rosa chinensis 'Old Blush' were identified using bioinformatics methods,and their phylogenetic relationships,gene structures,and expression patterns were analyzed as well. Phylogenetic tree of NUDIX was constructed from Rosa chinensis 'Old Blush',Rosa multiflora,Rosa rugosa,Rosa wichuraiana 'Basye's Thornless',Fragaria vesca,Malus domestica,Arabidopsis thaliana and Oryza sativa. It was found that the NUDIX can be classified into 13 subfamilies,among which the number of members of NUDIX1,NUDIX2,NUDIX4,and NUDIX11 subfamilies was greater than that of the other subfamilies. Collinearity analysis of genes revealed that the whole genome duplication event occurred in NUDIX gene family of Rosa chinensis 'Old Blush',resulting in two pairs of collinear genes. The chromosome localization results indicated that NUDIX genes of Rosa chinensis 'Old Blush' underwent multiple tandem duplication event,most of which went through purifying selection. Transcriptomic data analysis revealed that RcNUDIX1a/1b/1d/1e were specifically expressed in petals. RcNUDIX1b/1e/4c/9/11d might be involved in the stress response in leaf,RcIDI might participate in regulating young root and early floral development in plant tissues,and RcNUDIX2b/2d/2h might participate in the regulation of flower organ senescence. In conclusion,the quantity amplification of NUDIX genes in Rosa chinensis 'Old Blush' was caused mainly by tandem duplication event. The NUDIX gene family might play an important role in physiological processes such as secondary metabolism,growth and development,and stress response in leaf of Rosa chinensis 'Old Blush',which provides a theoretical basis for further research on the function of NUDIX genes in Rosa chinensis 'Old Blush'.
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