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2个杨梅品种果实及其蒸馏酒挥发性物质差异分析

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为比较杨梅主栽品种'荸荠'和优系'2010-70'果实基本品质指标和特征香气的差异,采用顶空固相微萃取-气相色谱-质谱法(headspace solid-phase microextraction-gas chromatography-mass spectrometry,HS-SPME-GC-MS)对'荸荠'2010-70'杨梅果汁及其一次蒸馏和二次蒸馏后所得杨梅酒进行挥发性物质成分及相对含量测定,并采用主成分分析(principal component analysis,PCA)和正交偏最小二乘判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA)对不同杨梅酒所含挥发性物质进行对比分析。结果显示:'2010-70'荸荠'果汁挥发性物质中特征香气物质β-石竹烯相对含量最高。杨梅蒸馏酒中萜类和酯类物质相对含量最高,在萜类物质中保留了杨梅果汁香气的α-石竹烯、β-石竹烯和(-)-异丁香烯等物质,在酯类物质中癸酸乙酯、苯甲酸苄酯和月桂酸乙酯相对含量较高。通过PCA、OPLS-DA发现,不同杨梅酒所含挥发性物质存在差异,其中2-己烯醛为差异最大的物质。结合感官评价可知,2个杨梅品种一次蒸馏后所得的挥发性物质总体优于二次蒸馏,'荸荠'一次和二次蒸馏后所得的挥发性物质总体优于'2010-70',但'2010-70'一次蒸馏酒的总体感官评价得分最高。不同杨梅品种和发酵蒸馏次数对杨梅酒中挥发性物质相对含量及风味影响较大,其中一次发酵蒸馏能较好体现杨梅酒的特色风味。
Differential analysis on volatile components of two varieties of Chinese bayberry fruits and their distilled liquors
The basic fruit quality indicators and characteristic aroma of the main Chinese bayberry variety 'Biqi' ('BQ') and a new selection '2010-70' were analyzed. Headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) was used to determine the volatile components of both 'BQ' and '2010-70' and the relative contents of their distilled liquors made from primary and secondary distillations. Principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) were used to compare and analyze volatile components in different Chinese bayberry distilled liquors. The results showed that the characteristic aroma β-caryophyllene in the volatile compounds of '2010-70' and 'BQ' juices had the highest content. The relative contents of terpenoids and esters were the highest in the Chinese bayberry distilled liquor. For terpenoids,α-caryophyllene,β-caryophyllene and (-)-isocaryophyllene in Chinese bayberry juice were retained,and the contents of ethyl decanoate,benzyl benzoate and ethyl laurate were relatively high in esters. The results of PCA and OPLS-DA showed a significant difference in the volatile components of the distilled liquors from the two Chinese bayberry varieties,with 2-hexenal being the most significant component. Combined with the sensory evaluation,for both varieties,the volatile components of the first distillation were generally better than those of the second distillation,and the volatile components of 'BQ' primary and secondary distillated liquor were generally better than those of '2010-70'. However,the '2010-70' primary distilled liquor had the best sensory evaluation. In conclusion,different Chinese bayberry varieties and fermentation and distillation times strongly influence the volatile component content and flavor of Chinese bayberry distilled liquor. Primary fermentation and distillation can better preserve the characteristic fruity flavor of Chinese bayberry in distilled liquor.

headspace solid-phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS)Chinese bayberry distilled liquorvolatile componentsdifferential analysis

陈竹韵、汪国云、周超超、赵岚、朱长青、贾惠娟、高中山

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浙江大学农业与生物技术学院果树科学研究所,浙江 杭州 310058

余姚市农业技术推广服务总站,浙江 宁波 315400

余姚市南方特色果树研究所,浙江 宁波 315400

顶空固相微萃取-气相色谱-质谱法 蒸馏型杨梅酒 挥发性物质 差异分析

2024

浙江大学学报(农业与生命科学版)
浙江大学

浙江大学学报(农业与生命科学版)

CSTPCD北大核心
影响因子:0.725
ISSN:1008-9209
年,卷(期):2024.50(6)