首页|中国东海及南海近海4采样点海水可培养细菌的多样性研究

中国东海及南海近海4采样点海水可培养细菌的多样性研究

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采用不同的培养方法从中国东海和南海近海水样品中分离得到105株细菌,探讨了不同海区可培养细菌的物种多样性和群落组成特点.16S rDNA序列分析结果表明,东海分离菌株分属于2个细菌门的14个种,南海分离菌株分属于3个细菌门的19个种.其中,东海分离菌株中α-变形菌占33.3%,γ-变形菌占57.2%,厚壁菌门细菌占9.5%.南海分离菌株中α-变形菌占41.9%,γ-变形菌占35.5%,拟杆菌门细菌占17.7%,厚壁菌门细菌占4.8%.东海和南海样品的分离菌株在种属水平上的多样性差异明显,后者的多样性指数高于前者.
Bacterial isolation and diversity analysis of four seawater sampling sites of the East China Sea and the South China Sea
A total of 105 strains were obtained from four sampling sites of the East China Sea and the South China Sea. Bacterial diversity and characteristics of bacteria communities were investigated. According to 16S rRNA gene sequences alignment, strains isolated from the East China Sea belong to 2 phyla and 14 species and strains isolated from the South China Sea belong to 3 phyla and 19 species. Strains isolated from the East China Sea were composed by γ-proteobactena (57. 2%), crproteobactena (33. 3%) and Firmicutes (9. 5 %). Strains isolated from the South China Sea composed by y-proteobactena (35. 5 %), orproteobactena (41.9%), Bacteroidetes (17.7%) and Firmicutes (4. 8%). Composition of isolated strains in genus and species level of the East China Sea and the South China Sea were significantly different. Microbial diversity of strains from the East China Sea was higher than that of the South China Sea.

bacteriacultivationdiversity

卢婧雯、张心齐、杜丽丽、杨志坚、吴敏、卢龙斗

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河南师范大学生命科学学院,河南新乡453007

浙江大学生命科学学院,浙江杭州310058

浙江大学舟山海洋研究中心,浙江舟山316021

细菌 可培养 多样性

舟山市科技计划项目(市校合作科技专项资金项目

10323

2012

浙江大学学报(理学版)
浙江大学

浙江大学学报(理学版)

CSTPCDCSCD北大核心
影响因子:0.709
ISSN:1008-9497
年,卷(期):2012.39(4)
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