首页|Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack

Co-culture of natural killer cells and tumor spheroids on a heterogeneous multilayer paper stack

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Multilayer paper-based cell culture,as an in vitro three-dimensional (3D) cell culture method,has been frequently used to research drug bioavailability,therapeutic efficacy,and dose-limiting toxicity in malignant tumors.This paper proposes a heterogenous multilayer paper stacking co-culture system to establish a model of natural killer (NK) cells moving through the endothelium layer and attacking tumor spheroids.This system consists of three layers:a bottom tumor-spheroid layer,a middle invasion layer,and a top endothelium layer.NK-92 cells were placed in the supernatant on top of the three layers.After two days of co-culture,the attack of tumor spheroids by NK cells was observed.We additionally examined the infiltration of NK-92 cells within the tumor spheroids at different Z-axis depths using a confocal microscope,and the results suggested that the system successfully realizes NK cells traveling cross the endothelium layer to form tumor-infiltrating NK cells (TINKs).The potential application of multilayer paper for co-culture models involving cancer cells and immune cells holds great promise for exploring the interaction dynamics of these two cell types.

Multilayer paper stackCo-cultureTumor spheroidHuman umbilical vein endothelial cell (HUVEC)Natural killer cell (NK cell)Migration

Yuanyuan XIE、Xiaoyan YANG、Rong PAN、Lixia GAO、Ling YU

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Key Laboratory of Luminescence Analysis and Molecular Sensing,Ministry of Education,Institute for Clean Energy and Advanced Materials,School of Materials and Energy,Southwest University,Chongqing 400715,China

National & Local Joint Engineering Research Center of Targeted and Innovative Therapeutics,College of Pharmacy & International Academy of Targeted Therapeutics and Innovation,Chongqing University of Arts and Sciences,Chongqing 402160,China

2024

10.1631/jzus.B2300617

浙江大学学报(英文版)(B辑:生物医学和生物技术)
浙江大学

浙江大学学报(英文版)(B辑:生物医学和生物技术)

CSTPCD
影响因子：0.798
ISSN：1673-1581
年,卷(期)：2024.25(12)