首页|仙鹤草内生真菌的分离鉴定与抗菌活性

仙鹤草内生真菌的分离鉴定与抗菌活性

Isolation and identification of endophytic fungi from Agrimonia pilosa Ledeb and pilot study of its antibacterial activity

扫码查看
植物内生真菌代谢产物丰富,作为一种寻找开发药物的重要资源已受到人们的日益关注.本实验对中药仙鹤草内生真菌进行分离,获得30株内生真菌,其中由根中分离得到17株,茎中分离得到7株,叶中分离得到6株.并采用形态学观察方法,对分离得到的内生真菌菌株进行初步鉴定,其分别归属于青霉属,顶孢霉属,镰孢霉属,根霉属,酵母属,珠网霉属,毛霉属和核盘菌属等8个真菌属.采用平板对峙法,研究各菌株对金黄色葡萄球菌的抗茵作用,通过初步筛选,共计9株表现抑菌活性.再进一步用内生真菌发酵萃取液做抑菌活性,发现仅3株菌株有抗茵活性,都属于镰孢霉属.为后续的活性物质分离奠定了基础.
Metabolites of Endophytic fungi as an important resource to find new bioactive compounds has received growing concern. In this paper, 30 endophytic fungi strains were isolated from roots, stems and leaves of Agrimonia pilosa Ledeb respectively, and morphological observation was employed to identify the endophytic fungi strains. The results showed that 30 endophytic fungi strains from Agrimonia pilosa Ledeb were Penicillium, Acremonium,Fusarium, Rhizopus, Saccharomyces, Arachniotus Schrot, Mucor, andSclerotinia. Meantime,effects of these Endophytic fungi strains on the growth of Staphylococcus aureus were investigated. The result indicated that 9 strains exerted antibacterial activity. Then, extracts of Endophytic fungi fermentation were used to treat Staphylococcus aureus, and the result indicated that only 3 strains of Fusarium exerted the antibacterial activity. Our results might be significant to study the bioactivity of endophytic fungi from Agrimonia pilosa Ledeb and find bioactive compounds in future.

陈笑笑、于海宁、应优敏、唐岚、李成平、单伟光

展开 >

浙江工业大学,药学院,浙江,杭州,310032

浙江树人大学,生物与环境工程学院,浙江,杭州,310015

仙鹤草 内生菌 分离 鉴定 抗菌活性

2011

浙江工业大学学报
浙江工业大学

浙江工业大学学报

CSTPCD
影响因子:0.704
ISSN:1006-4303
年,卷(期):2011.39(1)
  • 11
  • 13