双酚A降解菌的分离鉴定和降解特性研究
Isolation and characterization of a bacterium degrading bisphenol A
马云 1刘学虎 1洪骏1
作者信息
- 1. 浙江工业大学生物与环境工程学院,浙江杭州310014
- 折叠
摘要
从杭州市某污水处理厂的活性污泥中分离得到一株能高效降解双酚A的菌株.对其形态特征观察、生理生化特性研究和16S rDNA序列系统发育分析,确定该菌株为Shinella sp.HZB1.该菌株能以双酚A为惟一碳源和能源生长.考察温度,pH,微生物接种量以及它们交互作用对双酚A降解的影响,采用响应面法优化降解条件.当接种量为0.2 g/L时,最佳反应条件为温度32.7℃,pH7.5,质量浓度为100 mg/L的双酚A预测降解率达98.45％.最佳降解条件下,培养12h后,该菌株对质量浓度100 mg/L双酚A的降解率达到97.5％.双酚A为惟一碳源条件下,最大降解质量浓度达到300 mg/L.
Abstract
A novel strain,capable of degrading bisphenol A efficiently,was isolated from the activated sludge from a sewage plant in Hangzhou.Based on its 16S rDNA gene sequence analysis,morphological,physiological,and biochemical properties,the strain was identified as Shinella sp.HZB1.The bacterium could use bisphenol A as a sole source of carbon and energy for growth.Response surface methodology was chosen to optimize three important parameters (temperature,pH,inocula biomass)and their interactions which significantly affected the degradation of bisphenol A.With the fixed value of inocula biomass 0.2 g/L,the optimal conditions for the degradation of bisphenol A were 32.7 ℃,pH 7.5 and the predicted degradation rate was 98.45％.Bisphenol A (100 mg/L) was removed at rate of 97.5 ％ within 12 hour at the optimum condition.The high degradation concentration could be 300 mg/L with the sole source of carbon bisphenol A.
关键词
双酚A/申氏杆菌/生物降解/响应面Key words
bisphenol A/Shinella sp/biodegradation/response surface methodology引用本文复制引用
基金项目
国家自然科学基金资助项目(21007058)
浙江省自然科学基金资助项目(LY13B070007)
出版年
2014
NETL
NSTL
维普
万方数据