Study on the correlation between temperature,water activity and peanut aflatoxin pollution
In this paper,Zhanyou No.75 peanuts were used as raw materials to determine the contents of aflatoxin,ergosterol,and sterigmatocin in peanuts via high-performance liquid chromatography (HPLC).The expression of aflatoxin biosynthesis regulatory genes (aflS,aflR,aflO,and aflD ) was assessed using real-time qPCR.The study investigated the contamination of Aspergillus flavus and aflatoxin levels at varying water activity and storage temperatures.Results showed that water activity below 0.88 inhibited for Aspergillus flavus growth and aflatoxin.Optimal conditions for Aspergillus flavus growth were observed at 37 ℃ with a water activity of 0.96,while the highest aflatoxin levels occurred at 37 ℃ and a water activity of 0.94.Although lower temperature (28 ℃)promoted the expression of aflatoxin biosynthesis genes,the overall growth of Aspergillus flavus was inhibited,leading to reduced aflatoxin production.At 37 ℃,despite lower expression of the gene aflR,robust fungal growth contributed to higher aflatoxin.The study also identified a correlation between sterigmatocin and aflatoxin,suggesting that monitoring the sterigmatocin could serve as an early warning of aflatoxin contamination.These findings provide a new direction and theoretical support for the prevention and control of peanut aflatoxin pollution in peanut.
万方数据
维普
