Determination of Cartap Residue in Plant-Based Foods by Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry
Using triple quadrupole liquid chromatography-mass spectrometry technology,qualitative and quantitative analysis of cartap in different plant-based foods was carried out.By screening and optimizing the extraction and purification conditions of cartap,a rapid,convenient,accurate and efficient analysis method for cartap in plant-based foods was established.The chromatographic column was ZORBAX SB-C18(2.1 mm×100 mm,3.5 μm),the mobile phase was water(0.1%formic acid)and methanol,the gradient elution flow rate was 0.3 mL/min,the multi reaction monitoring mode(MRM)was used,the positive ion mode was select-ed for the electric spray ion source(ESI),and the quantitative ion pair was 238.1/149.9.The sample was ex-tracted with 2%acetic acid acetonitrile,purified by QuEChERS method,directly measured after membrane filtration,and quantified by external standard method.The results showed that the linear relationship of the target substance was good within the range of 5～200 ng/mL,with a determination coefficient(R2)of 0.9992.The recovery rate of the sample spiked was 72%～86%,and the relative standard deviation of the precision test was 0.7%～6.3%.This method has wide applicability,convenient operation,good recovery rate,and high precision,and can be used for the determination of residual cartap in plant-based foods.
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