Objective:To optimize the extraction process of Mistletoe by using the Box-Behnken design-response surface method,and the comprehensive content of the two active ingredients as the evaluation index.Methods:The ethanol volume fraction,extraction time and solid-liquid ratio of Mistletoe extraction process were optimized by using the Box-Behnken design-response surface method on the basis of single factor.Ultra performance liquid chromatography(UPLC)was used to determine the content of two active components in Mistletoe from 14 origins.Results:The optimal extraction process of Mistletoe was 71%ethanol-aqueous solution,liquid-solid ratio 25:1,and extraction time 67 min.The optimal extraction process showed that the average comprehensive content was 571.7 μg·g-1 when the weight ratio of Syringin to homoeriodictyol-7-O-β-D-glucoside was 7:3.The contents of Syringin and homoeriodictyol-7-O-β-D-glucoside ranged from 128.7～662.8 μg·g-1 and 77.24～801.8 μg·g-1.The contents of Syringin and homoeriodictyol-7-O-β-D-glucoside in Mistletoe from different origins were different,among which the content of homoeriodictyol-7-O-β-D-glucoside in Mistletoe from Sichuan was relatively high,which was 0.84 mg·g-1,and the content of Syringin from Mistletoe from Anhui was relatively high at 0.47 mg·g-1.Conclusion:The method established in this study has high sensitivity,good separation effect,stable and reliable results,which can provide a scientific basis for the extraction of active ingredients,quality evaluation and resource development and utilization of Mistletoe.
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