Influence of ecological factors on the genetic diversity of the population of Akebia trifoliata and Akebia quinata
Genetic diversity of 34 species of Akebia trifoliata(Thunb.)Koidz and Akebia quinata(Thunb.)Decne from seven regions in Zhejiang Province was analyzed using inter simple sequence repeat(ISSR)amplification technology and rDNA internal transcribed spacer(ITS)sequence analysis technology.The results showed that the cluster analysis of Akebia quinata(Thunb.)Decne was significantly correlated with altitude,but less correlated with the soil organic matter content and pH value.The 15 primers selected were used in the ISSR analysis for Akebia trifoliata(Thunb.)Koidz to obtain 265 DNA bands,of which 98.49%bands were polymorphic.The cluster analysis indicated that Akebia trifoliata(Thunb.)Koidz from the same region could be classified as one cluster respectively.And the genetic differences were significant in different regions.The mean Nei's gene diversity index(h)of Akebia trifoliata(Thunb.)Koidz was 0.503 3 and Shannon's information diversity index(I)was 0.689 0,which showed rich genetic diversity of Akebia trifoliata(Thunb.)Koidz.The correlation analysis showed that it wasn't significantly correlated between the three ecological factors of altitude,soil organic matter content and pH value and genetic diversity of Akebia trifoliata(Thunb.)Koidz.In summary,rDNA ITS sequence analysis and ISSR molecular marker were applied separately to genetic diversity analysis of Akebia trifoliata(Thunb.)Koidz and Akebia quinata(Thunb.)Decne.The study indicates that altitude was significantly correlated with genetic diversity of Akebia quinata(Thunb.)Decne.Genetic diversity of Akebia trifoliata(Thunb.)Koidz wasn't significantly correlated with altitude,soil organic matter content and pH value,but genetic differences of Akebia trifoliata(Thunb.)Koidz in different regions was significant.
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NSTL
万方数据
维普
