摘要
液泡转化酶抑制蛋白(vacuolar invertase inhibitor,VIF)在调节植物蔗糖代谢,调控生长发育及响应逆境等方面发挥重要作用.本试验从番茄 Alisa 中克隆到 VIF 基因(SlVIF)和 cDNA 序列.结果显示,SlVIF基因与 cDNA序列全长均为 528 bp,不含有内含子,共编码 175 个氨基酸.SlVIF 蛋白分子量为 19.92 ku,理论等电点为 5.53,含有一个信号肽序列,保守结构域为 PMEI.系统进化关系表明,茄科物种的 VIF 亲缘关系最近,且基序具有一致性.组织特异性表达结果显示,在不同的番茄材料中,SlVIF 的表达模式存在差异,在Heinz番茄中,其在根部有高水平的表达;在 LA1589 番茄中,其在果实发育的各阶段,尤其是在成熟果实及根部有高水平的表达.说明 SlVIF可能通过介导蔗糖代谢影响果实成熟并调控根系生长.SlVIF 受到水分胁迫的诱导,响应非生物胁迫过程.
Abstract
Vacuolar invertase inhibitor(VIF)plays an important role in regulating higher plant sucrose metabolism and responding to abiotic stress.In this study,VIF gene and cDNA sequence were cloned from Alisa.The results indicated that the full length of SlVIF gene and cDNA was 528 bp,without intron region,encoding 175 amino acids.The molecular weight of SlVIF protein was 19.92 ku,and the theoretical isoelectric point was 5.53.It contained a signal peptide sequence,and the conservative domain was PMEI.The phylogenetic relationship exhibited that the VIF of Solanaceae species was the closest and the motifs were consistent.The tissue specific expression results showed that it had different expression patterns in various tomato materials.In Heinz,it had high transcription level in roots.In LA1589,it was highly expressed in all stages of fruit development,especially in mature fruits and roots,which indicated that SlVIF gene may play a role in influencing fruit ripening and regulating root growth by mediating sucrose metabolism.SlVIF was induced by water stress and responded to abiotic stress processes.
基金项目
浙江省重点研发计划(2021C02052)
国家重点研发计划(2018YFD1000800)
国家自然科学基金面上项目(32172555)
国家自然科学基金面上项目(31772294)
浙江省农业(蔬菜)新品种选育科技重大专项(2021C02065)
现代农业产业技术体系建设项目(CARS-23-G44)
NETL
NSTL
维普
万方数据