Analysis of chloroplast genome structure and evolutionary development of Metaplexis japonica
Metaplexis japonica(Thunb.)Makino has certain edible and medicinal value.The purpose of this study is to obtain the chloroplast genome splicing of Metaplexis japonica,analyze the sequence characteristics of the chloroplast genome,and determine its phylogenetic relationship in related species.The research method is to use the Illumina platform for high-throughput sequencing of the chloroplast genome of Metaplexis japonica,using a series of bioinformatics analysis methods to complete genome splicing and gene annotation,and analyze repeat sequence characteristics.Combining multiple closely related species of Metaplexis japonica,analyzing the variation of boundary genes,a multi species phylogenetic tree was constructed.The results showed that the chloroplast genome of Metaplexis japonica is a typical circular tetrad structure,with a total length of 157 081 bp and a GC content of 36.49%.A total of 132 genes were annotated,including 45 photosynthesis related genes,74 self replication related genes,6 other genes,and 7 functionally unknown genes.In total,271 simple repeat sequence(SSR)loci were identified,with single base repeats being the main sequence,followed by triple base repeats.Multiple species comparison and phylogenetic analysis have confirmed that Metaplexis japonica belongs to the genus Cynanchum,and shows a closer relation to the genera Asclepias and Calotropis.
NETL
NSTL
万方数据
维普
