Genetic diversity analysis and control strategies of weedy rice in Huzhou Area
Weedy rice,as a genetically close weed of rice,is one of the most difficult weeds to control in rice fields.With the advancement of modern agriculture,the area of rice direct seeding has increased year by year,and the harm of weed rice to rice yield has become increasingly serious.In this study,48 pairs of SSR primers specified in NY/T 1433-2014 were used to analyze the genetic diversity of 15 weed rice plants collected from Huzhou City,and their phenotypic traits were analyzed and compared.The results showed that the phenotypic differences of the 15 weed rice plants were significant,and the grain drop was significantly stronger than that of the control Jietian rice 001 and Wuyunjing.The SSR results showed that a total of 104 alleles were detected by 48 pairs of primers,and the average polymorphic information was 0.313 8,showing moderate polymorphism.The average number of alleles was 2.312 5,the average number of effective alleles was 1.577 2,and the average Shannon information index was 0.515 4,indicating high genetic diversity.In this study,ALS gene sequence analysis was performed on these 15 weedy rice lines,and 32 SNP loci were found,but none of them produced resistance.Therefore,the control strategy of weedy rice in Huzhou was preliminarily discussed by using rice resistant to ALS inhibitor herbicides.The results showed that the weeds found in Huzhou could be controlled by spraying ALS inhibitor herbicides.These results will provide a reference for the control strategy of weed rice in Huzhou.
NETL
NSTL
万方数据
维普
