UPLC-MS/MS based technique for the detection of diuron residues in soil
In order to improve the extraction efficiency of diuron in soil and to detect its residues more accurately,pressurized solvent extraction was used as the extraction method for diuron in soil,and a new method for the extraction and detection of diuron in soil was established by using ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry.The sample was freeze-dried and loaded into a pressurized solvent extractor,and a methanol-dichloromethane mixture was used as the extractant.The extract was cleaned up by a Carb/PSA graphitized carbon black/ethylenediamine-N-propyl solid phase extraction column,and then separated by a C18 column with a gradient elution of methanol-dichloromethane mixture at a volume ratio of 1 ∶ 9.The electron bombardment ion source and multiple reaction were selected in the mass spectrometry monitoring mode.The results showed that there was a good linear relationship within the range of 20-500 μg·L-1 diuron,with a correlation coefficient of 0.999 5,and the target peak separation effect is good.The spiked recoveries of diuron were 71.2%-90.3%,the relative standard deviations were 1.7%-9.4%,and the detection limits were 0.000 287 0 μg·g-1.This method is conducive to the operation of large-scale detection of diuron residues in soil,improving the extraction and detection efficiency of target substances,and improving the accuracy of sample detection.
diuronpressurized solvent extractionliquid mass spectrometrypesticide residues
NETL
NSTL
万方数据
维普
