首页|外源GA3和ABA对烤烟内源激素调控效应和常规化学成分的影响

外源GA3和ABA对烤烟内源激素调控效应和常规化学成分的影响

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本文从激素和基因水平探究外源激素赤霉素(GA3)和脱落酸(ABA)的作用机理，研究其对烤烟生长发育及产量和质量的影响，为田间施用外源激素提供理论依据。采用大田试验，材料为中烟 100(ZY100)和NC71，喷施不同浓度配比的GA3 和ABA，测定不同时期烟株的农艺性状、基因表达量、生理特性、内源激素含量及烤后烟叶化学成分。结果表明，与未喷施 GA3 处理相比，喷施 GA3 后 ZY100 株高升高 20%，NC71 株高升高31%;喷施ABA后ZY100 株高降低 17%，NC71 株高降低 10%。喷施GA3 后ZY100 和NC71 内源赤霉素含量分别升高 20%和 30%;喷施外源GA3 后ZY100 和NC71 烟株内 CPS、GA20ox和 PSY 的基因表达量均上调，GA2ox基因表达量下调。喷施GA3 提高了ZY100 和NC71 烟株体内叶绿素以及可溶性蛋白的含量，降低了丙二醛含量，提高了烤后烟叶总糖含量、钾含量、钾氯比，降低了烟碱含量和氯含量，显著提高了烤烟的经济性状。
Effects of exogenous GA3 and ABA on endogenous hormone regulation and conventional chemical composition of flue-cured tobacco
In this paper,the mechanism of exogenous hormones gibberellin(GA3)and abscisic acid(ABA)was investigated from the hormone and gene levels,and the effects of exogenous hormones on the growth and development,yield and quality of flue-cured tobacco were studied,providing theoretical basis for field application of exogenous hormones.Field experiments were conducted with Zhongyan100 and NC71,and different concentrations of GA3 and ABA were sprayed to determine the agronomic traits,gene expression,physiological characteristics,endogenous hormone content and chemical composition of tobacco leaves after roasting.The results showed that the height of ZY100 and NC71 plants increased by 20%and 31%respectively after GA3 spraying compared with no GA3 spraying.After spraying ABA,the height of ZY100 plant decreased by 17%,and the height of NC71 plant decreased by 10%.After spraying GA3,the content of endogenous gibberellin in ZY100 and NC71 increased by 20%and 30%,respectively.After spraying exogenous GA3,the gene expressions of CPS,GA20ox and PSY in ZY100 and NC71 tobacco plants were up-regulated,while the gene expressions of GA2ox were down-regulated.Spraying GA3 increased the contents of chlorophyll and soluble protein in ZY100 and NC71,decreased the content of malondialdehyde,increased the total sugar content,potassium content,potassium chlorine ratio,decreased the nicotine content and chlorine content,and significantly improved the economic properties of flue-cured tobacco.

gibberellinabscisic acidflue-cured tobaccogene expressionphysiological characteristicsconventional chemical composition

李波、陈小翔、张文军、翟争光、刘化冰、江智敏、徐志强

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浙江中烟工业有限责任公司 技术开发分公司, 浙江 杭州 310008

湖南省烟草公司 长沙市公司, 湖南 长沙 410000

赤霉素 脱落酸 烤烟 基因表达 生理特性 常规化学成分

中国烟草总公司河南省公司重点项目河南省烟草公司许昌市公司重点项目贵州省烟草公司贵阳市公司重点项目湖南省烟草公司长沙市公司重点项目广西壮族自治区烟草公司百色市公司重点项目福建省烟草公司南平市公司重点项目

2021410000240021202041100024006921-23A04202145100020165NYK2021-09-03

2024

10.16178/j.issn.0528-9017.20221178

浙江农业科学
浙江省农业科学院,浙江大学

浙江农业科学

影响因子：0.523
ISSN：0528-9017
年,卷(期)：2024.65(1)
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