摘要
以总黄酮、总多酚、总多糖含量及超高效液相色谱-四级杆飞行时间串联质谱法(UPLC-Q-TOF-MS/MS)成分分析、1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、2,2′-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除能力为评价指标,对浙江省杭州市富阳区内发现的 5 个三叶青种源进行检测,发现石硃 1、4、5 号总多糖、总黄酮、总酚含量明显高于石硃 2、3 号;但 DPPH、ABTS 自由基清除能力以石硃 4、5号为好.研究结果可为富阳区内三叶青种质资源分类、保存、鉴定及评价提供基础理论支撑.
Abstract
Based on the content of total flavonoids,total polyphenols and polysaccharides and the UPLC-Q-TOF-MS/MS component analysis,five provenances of Tetrastigma hemsleyanum found in Fuyang District,Hangzhou City,Zhejiang Province were detected with DPPH radical scavenging ability and ABTS radical scavenging ability as evaluation indexes.It was found that the total polysaccharides,flavonoids,and phenols content of Shizhu 1,4,and 5 were significantly higher than those of Shizhu 2 and 3,But the free radical scavenging ability of DPPH and ABTS was better than that of Shizhu 4 and 5.The results could provide basic data support for the classification,preservation,identification and evaluation of Tetrastigma hemsleyanum germplasm resources in Fuyang District.
维普
万方数据