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激素和糖分对暗紫贝母生根培养的影响

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本实验以苗龄为 90 d的暗紫贝母芽繁苗为实验材料,在组培生根培养过程中添加不同浓度的激素和糖分,研究其对暗紫贝母生根的影响,筛选出暗紫贝母的最适生根培养基。结果表明:在生根培养基为基本培养基(MS)+9。4 g·L-1 卡拉胶(car)+0。5 mL·L-1 萘乙酸(NAA)+0。5 mL·L-1 吲哚丁酸(IBA)+30 g·L-1 蔗糖时暗紫贝母生根情况最好,生根率高达 81。3%,根系粗壮,且有效根数最多,平均每株有 3。4 条根系,且没有被污染。本研究为以后建立暗紫贝母组培快繁体系、大规模标准化生产优良暗紫贝母种苗提供参考。
Effects of hormones and sugar on rooting culture of Fritilaria unibracteata Hsiao et K.C.Hsia
In this study,the 90-day-old buds of Fritilaria unibracteata Hsiao et K.C.Hsia were used as experimental materials,and different concentrations of hormones and sugars were added to study their effects on the rooting of Fritilaria unibracteata Hsiao et K.C.Hsia in tissue culture,and the most suitable rooting medium for Fritilaria unibracteata Hsiao et K.C.Hsia was screened.The results showed that the rooting medium was MS+9.4 g·L-1 car+0.5 mL·L-1 NAA+0.5 mL·L-1 IBA+30 g·L-1 sucrose had the best rooting effect,with a rooting rate of 81.3%,a thick root system,and the largest number of effective roots,with an average of 3.4 roots per plant,and no pollution.This study provides a reference for the establishment of a fast propagation system for tissue culture and large-scale standardized production of excellent Fritilaria unibracteata Hsiao et K.C.Hsia seedlings in the future.

Fritilaria unibracteata Hsiao et K.C.Hsiatissue culturehormonessugarrooting

字胡润、赵艳娟、管世超、苏娟、文凤、张玉蓉、王晓、李靖瑞、岳健

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云南青谷生物科技有限公司,云南 昆明 650506

云南山里红生物科技有限公司,云南 昆明 650229

暗紫贝母 组织培养 激素 糖分 生根

2024

浙江农业科学
浙江省农业科学院,浙江大学

浙江农业科学

影响因子:0.523
ISSN:0528-9017
年,卷(期):2024.65(2)
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