激素和糖分对暗紫贝母生根培养的影响

Effects of hormones and sugar on rooting culture of Fritilaria unibracteata Hsiao et K.C.Hsia

字胡润 ¹赵艳娟 ²管世超 ¹苏娟 ²文凤 ¹张玉蓉 ¹王晓 ²李靖瑞 ¹岳健³

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作者信息

1. 云南青谷生物科技有限公司,云南昆明 650506
2. 云南山里红生物科技有限公司,云南昆明 650229
3. 云南青谷生物科技有限公司,云南昆明 650506;云南山里红生物科技有限公司,云南昆明 650229
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摘要

本实验以苗龄为 90 d的暗紫贝母芽繁苗为实验材料,在组培生根培养过程中添加不同浓度的激素和糖分,研究其对暗紫贝母生根的影响,筛选出暗紫贝母的最适生根培养基.结果表明:在生根培养基为基本培养基(MS)+9.4 g·L-1 卡拉胶(car)+0.5 mL·L-1 萘乙酸(NAA)+0.5 mL·L-1 吲哚丁酸(IBA)+30 g·L-1 蔗糖时暗紫贝母生根情况最好,生根率高达 81.3％,根系粗壮,且有效根数最多,平均每株有 3.4 条根系,且没有被污染.本研究为以后建立暗紫贝母组培快繁体系、大规模标准化生产优良暗紫贝母种苗提供参考.

Abstract

In this study,the 90-day-old buds of Fritilaria unibracteata Hsiao et K.C.Hsia were used as experimental materials,and different concentrations of hormones and sugars were added to study their effects on the rooting of Fritilaria unibracteata Hsiao et K.C.Hsia in tissue culture,and the most suitable rooting medium for Fritilaria unibracteata Hsiao et K.C.Hsia was screened.The results showed that the rooting medium was MS+9.4 g·L-1 car+0.5 mL·L-1 NAA+0.5 mL·L-1 IBA+30 g·L-1 sucrose had the best rooting effect,with a rooting rate of 81.3％,a thick root system,and the largest number of effective roots,with an average of 3.4 roots per plant,and no pollution.This study provides a reference for the establishment of a fast propagation system for tissue culture and large-scale standardized production of excellent Fritilaria unibracteata Hsiao et K.C.Hsia seedlings in the future.

关键词

暗紫贝母/组织培养/激素/糖分/生根

Key words

Fritilaria unibracteata Hsiao et K.C.Hsia/tissue culture/hormones/sugar/rooting

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出版年

2024

浙江农业科学

浙江省农业科学院,浙江大学

浙江农业科学

影响因子：0.523

ISSN：0528-9017

参考文献量21

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