Application of high-throughput sequencing technology in the study of microbial diversity of medicinal plants
Medicinal plants have always been a rich resource pool for producing natural products,which have broad medical and medicinal prospects.Beneficial microorganisms of medicinal plants have significant effects on their growth and development,stress resistance,synthesis and accumulation of secondary metabolites.Due to the limited factors such as excessive picking and destruction of living environment of medicinal plants,the resources of wild medicinal materials are scarce.Therefore,a large number of medicinal plant resources can only be obtained through artificial cultivation and cultivation,but artificial cultivation cannot simulate the real growing environment of wild medicinal materials.Studies have shown that to improve the yield and quality of medicinal plants and realize the application potential of beneficial microorganisms of medicinal plants,it is very necessary to study medicinal plant microorganisms.High-throughput sequencing technology has greatly changed many areas of research on medicinal plant microorganisms.It has become a hot field for researchers to explore the microbial diversity of medicinal plants using high-throughput sequencing technology.In this paper,through the summary of relevant literature,the research progress of the application of high-throughput sequencing technology in four aspects of microbial species diversity,structural diversity,functional diversity and genetic(gene)diversity of medicinal plants in recent years was reviewed,and the existing problems and development trends of high-throughput sequencing technology in its application were summarized and prospected.It provides reference for our subsequent understanding of microbial diversity of medicinal plants.