首页|烟草抗感南方根结线虫品种根部差异表达mRNA的PPI网络分析

烟草抗感南方根结线虫品种根部差异表达mRNA的PPI网络分析

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基于品种间的差异表达基因，选取烟草抗病品种 K326 与感病品种长脖黄为试验材料，利用生物信息学分析方法筛选出与抗感染相关的关键基因，通过蛋白互作网络分析(Protein-Protein Interaction，PPI)以及Gene Ontology(GO)功能分类分析(包括 Biological Process分析与 KEGG 通路分析)对差异表达基因进行生物学功能注释，并找出 PPI网络中的重要基因以及作用通路一致的基因，并对这些基因加以解析，以明确抗南方根结线虫烟草品种抗性机制，为研究烟草抗南方根结线虫的分子机制提供理论依据。结果表明，差异表达基因主要在细胞代谢、胞内体转运、RNA 介导的基因沉默等 9 条与抗感染相关的通路中显著富集，并且筛选出Fab1b、Fab1c、Fab1d、Glu1、Dcl1、Dcl2、Dcl4 共 7 个同时构成 PPI网络的关键基因。
PPI network analysis of differentially expressed mRNA in the roots of tobacco varieties resistant to Meloidogyne incongnita(Kofold&White)Chitwood
Based on the differentially expressed genes among varieties,tobacco disease resistant variety K326 and susceptible variety Changbohuang were selected as experimental materials,and key genes related to infection resistance were screened using bioinformatics analysis methods,this article used Protein Protein Interaction(PPI)and Gene Ontology(GO)functional classification analysis(including Biological Process analysis and KEGG pathway analysis)to annotate the biological functions of differentially expressed genes,identify important genes and genes with consistent pathways in the PPI network,analyze these genes to clarify the resistance mechanism of tobacco varieties to Meloidogyne incongnita(Kofold&White)Chitwood,and provide theoretical basis for studying the molecular mechanism of tobacco resistance to Meloidogyne incongnita(Kofold&White)Chitwood.The results showed that differentially expressed genes were significantly enriched in 9 pathways related to anti-infection,including cell metabolism,intracellular transport,and RNA mediated gene silencing.Seven key factor genes that simultaneously formed the PPI network were identified,including Fab1b,Fab1c,Fab1d,Glu1,Dcl1,Dcl2 and Dcl4.

tobaccoMeloidogyne incongnita(Kofold&White)Chitwoodprotein protein interactiongene ontology(GO)functional classification analysiskey genes

陈小翔、张文军、翟争光、徐志强、刘化冰、钟永健、江智敏

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浙江中烟工业有限责任公司 技术开发分公司,浙江 杭州 310008

湖南省烟草公司 长沙市公司,湖南 长沙 410000

烟草 南方根结线虫 PPI网络分析 GO分类 关键基因

中国烟草总公司河南省公司重点项目河南省烟草公司许昌市公司重点项目贵州省烟草公司贵阳市公司重点项目湖南省烟草公司长沙市公司重点项目广西壮族自治区烟草公司百色市公司福建省烟草公司南平市公司

202141000024002120204110002400692020-0721-23A04202145100020165NYK2021-09-03

2024

10.16178/j.issn.0528-9017.20221179

浙江农业科学
浙江省农业科学院,浙江大学

浙江农业科学

影响因子：0.523
ISSN：0528-9017
年,卷(期)：2024.65(2)
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