Based on the differentially expressed genes among varieties,tobacco disease resistant variety K326 and susceptible variety Changbohuang were selected as experimental materials,and key genes related to infection resistance were screened using bioinformatics analysis methods,this article used Protein Protein Interaction(PPI)and Gene Ontology(GO)functional classification analysis(including Biological Process analysis and KEGG pathway analysis)to annotate the biological functions of differentially expressed genes,identify important genes and genes with consistent pathways in the PPI network,analyze these genes to clarify the resistance mechanism of tobacco varieties to Meloidogyne incongnita(Kofold&White)Chitwood,and provide theoretical basis for studying the molecular mechanism of tobacco resistance to Meloidogyne incongnita(Kofold&White)Chitwood.The results showed that differentially expressed genes were significantly enriched in 9 pathways related to anti-infection,including cell metabolism,intracellular transport,and RNA mediated gene silencing.Seven key factor genes that simultaneously formed the PPI network were identified,including Fab1b,Fab1c,Fab1d,Glu1,Dcl1,Dcl2 and Dcl4.
关键词
烟草/南方根结线虫/PPI网络分析/GO分类/关键基因
Key words
tobacco/Meloidogyne incongnita(Kofold&White)Chitwood/protein protein interaction/gene ontology(GO)functional classification analysis/key genes
维普
万方数据