摘要
通过室内毒力测定及大田试验进行药剂筛选,筛选出对甘薯茎腐病的有效防控药剂,以期为甘薯茎腐病的防治提供新的途径.采用含毒介质法中的最低抑制浓度法,对 19 种药剂进行室内毒力测定,并在此基础上选取 6 种防治效果较好的药剂开展田间防治试验.结果表明,室内毒力测定中溴硝醇的抑菌效果最强,抑菌最低有效浓度为 4.69 mg·L-1,其次为噻霉酮、中生菌素、春雷霉素(加收米)、土霉素、硫酸链霉素、春雷·噻唑锌,抑菌最低有效浓度分别为 7.50、18.25、28.13、37.50、75.00、150.00 mg·L-1.大田试验选取的 6种药剂中,春雷·噻唑锌的防治效果最佳,可达 82.16%,其次噻霉酮、中生菌素、溴硝醇和春雷·中生的防治效果分别为 79.55%、77.18%、76.56%、75.02%,均可作为田间甘薯茎腐病的有效防控药剂.
Abstract
In order to provide new ways for the prevention and control of stem rot disease in Dioscorea esculenta,this article conducts drug screening through indoor toxicity testing and field experiments to screen effective prevention and control drugs for stem rot disease of Dioscorea esculenta.Using the minimum inhibitory concentration method in the toxic medium method,the indoor toxicity of 19 pesticides was determined,and based on this,6 pesticides with better control effects were selected for field control experiments.The results showed that bromonitrol had the strongest antibacterial effect in indoor toxicity testing,with a minimum effective concentration of 4.69 mg·L-1,followed by thiamethoxazole,mesobiotin,amphotericin(kasumin),oxytetracycline,streptomycin sulfate,and amphotericin·thiazole zinc.The minimum effective concentrations of inhibition were 7.50,18.25,28.13,37.50,75.00 and 150.00 mg·L-1,respectively.Among the six selected pesticides in the field experiment,amphotericin·thiazole zinc had the best control effect,with a control effect of 82.16%,followed by thiamethoxazole,mesobiotin,bromonitrol,and amphotericin·zhongshengmycin,with a control effect of 79.55%,77.18%,76.56%,and 75.02%,respectively.They can all be used as effective control agents for stem rot disease of Dioscorea esculenta in the field.
基金项目
浙江省重点研发计划(2017C02002)
浙江省农科院省部共建农产品质量安全危害因子与风险防控国家重点实验室自主设计项目(2010DS700124-ZZ2014)