Identification,evaluation and screening for low temperature and low light tolerance in germplasm resources of sponge gourd
With the continuous development of facility agriculture,there is an urgent need to select and breed new sponge gourd varieties with low temperature and low light tolerance.Identification and evaluation of low temperature and low light tolerance of germplasm resources can lay an important material foundation for new variety selection and breeding.In this study,192 sponge gourd germplasm resources were evaluated for low temperature and low light tolerance in an intelligent artificial climate chamber with simulating low temperature and low light environment.The cold damage classification,cold injury index and relative conductivity for each germplasm resource were identified and evaluated.The results showed that the cold injury index of 192 sponge gourd germplasm resources ranged from 35.00%to 92.86%,with an average of 61.98%and a coefficient of variation of 19.50%;the relative conductivity ranged from 10.39%to 82.00%,with an average of 49.18%and a coefficient of variation of 24.80%.Combining with the cold injury index(≤45%)and relative conductivity(≤30%),11 sponge gourd germplasm resources with low temperature and low light tolerance were screened.Correlation analysis of cold injury index(y)and relative conductivity(x)of 192 sponge gourd germplasm resources were carried out,and the two parameters were highly significantly and positively correlated.Additionally,the linear equation y=0.892x+0.1811(R2 =0.8101)was obtained.The results of the study provided important theoretical guidance and material basis for low temperature and low light tolerance identification,evaluation,selection and new varieties breeding in sponge gourd.
sponge gourdgermplasm resourceslow-temperature and low-lightcold injury indexrelative electric conductivity