Genetic diversity analysis of 64 white-fruited pepper germplasm resources
White-fruited pepper is the most regionally characteristic type of pepper(capsicum)in Zhejiang Province,understanding its genetic diversity serves as an essential foundation for promoting the breeding of new varieties of white-fruited pepper.In this study,64 white-fruited pepper inbred lines collected from China and abroad were sequenced by Hyper-seq.Principal component analysis,cluster analysis and population structure analysis were also performed.The results showed that a total of 3511122 single nucleotide polymorphism(SNP)variation and 922513 insertion-deletion(InDel)were identified from 12 chromosomes of pepper.The average number of SNPs and InDels per chromosome was 292593.5 and 76876.0,respectively.The annotation results revealed that InDel and SNP variation predominantly manifested in the intergenic regions,reaching a quantity of 4000310.The next significant occurrence was within intronic regions,totaling 226301.Cluster analysis and population structure analysis,based on SNP diversity,classified the 64 white pepper materials into two main groups:one comprised 36 fresh white-fruited pepper and 1 ornamental white-fruited pepper,while the other consisted of 27 ornamental white-fruited pepper.The principal component analysis results indicated that two groups clustered distinctly in the PC1 and PC2 regions.This study unveils the genetic structure and genetic diversity of white-fruited pepper germplasm resources,providing a foundational understanding for the future development and application of white-fruited pepper germplasm.