Identification of CHS gene in Chinese cabbage and transcriptional expression analysis under high nitrogen levels
In order to investigate the mechanism of exacerbation of Chinese cabbage petiole black spot disease caused by high nitrogen levels,the resistant and suscetible Chinese cabbage were treated with normal and high nitrogen levels,and sampled petioles were sampled at different time before and after treatment for transcriptome sequencing.The Chinese cabbage chalcone synthase(CHS)gene was identified and analyzed for different Chinese cabbage CHS genes at normal and high nitrogen levels.The differential expression between resistant and susceptible Chinese cabbage showed that a total of 7 CHS genes were identified,of which 3 genes(BrCHS1,BrCHS3,and BrCHS4)had higher expression levels under high nitrogen levels than the normal nitrogen levels,and the expression level of susceptible Chinese cabbage was higher than that of resistant Chinese cabbage under high nitrogen levels.Therefore,it is speculated that these three Chinese cabbage CHS genes may be related to the formation of Chinese cabbage petiole black spot disease.This study lays the foundation for revealing the mechanism of black spot disease on the petiole of Chinese cabbage.
Chinese cabbagepetiole black spot diseasechalcone synthetasehigh nitrogen levelexpression analysis
NETL
NSTL
万方数据
维普
