Identification of the purity of Dianhu 1 hybrids using InDel markers
Dianhu 1 is a new variety of Brassica rapa L.ssp.chinensis L.bred by Ningbo Weimeng Seed Industry Co.Ltd.,but the speed of seed purity identification has limited the promotion of this variety.In order to realize high-throughput and rapid identification of the purity of hybrid seeds of this variety,InDel markers for purity detection were developed on the basis of the resequencing of the genomes of the parents of Dianhu 1(Male parent:EPG-194;Female parent:EPG-168).The DNA samples of Dianhu 1 and its parents were used to select primers D1-7 with complementary band types,clear amplification bands and fast separation speed,and the primers were used to detect the F1 population of Dianhu 1 in the field.The primer D1-7 was used to test the F1 population of Dianhu 1 in the field(400 plants for EpG-194×EpG-168 group and 400 plants EpG-168×EpG-194 group).In the experiment,the molecular detection results of primer D1-7 showed that there were 146 and 32 non-F1 hybrids in the EpG-194×EpG-168 group and EpG-168×EpG-194 group,and 146 and 32 non-F1 hybrids in the EpG-194×EpG-168 group and EpG-168×EpG-194 group were identified by field phenotyping,which were identical to the results of the 800 single plants identified by the two primers.The results showed that the InDel primers D1-7 accurately identified the mixed maternal selfed hybrids in Dianhu 1 population,which laid the foundation for the rapid identification of the purity of Dianhu 1 hybrids in the future.
NETL
NSTL
万方数据
维普
