Optimization of regeneration in vitro system of Anoectochilus roxburghii
In order to obtain the optimal rapid propagation system,we studied the effects of plant hormone combinations and ratios on the induction,proliferation and rooting of adventitious shoots.The results showed that exogenous hormones had a significant effect on the induction of cluster buds.The optimal culture combination for bud induction was MS+0.1 μmol·L-1 NAA+20 μmol·L-1 6-BA.The optimal culture combination for rooting induction was 1/2 MS+10 μmol·L-1 IBA+2.0 g·L-1 activated carbon.In the mixed culture medium with a volume ratio of 3∶1∶1 for peat,perlite and vermiculite,the growth of regenerated seedlings was the best,and the survival rate was more than 90%.This study provide technical support for further optimization of the scale breeding of Anoectochilus roxburghii.
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