Study on tissue culture and rapid propagation of Hydrangea macrophylla cv.Ivetta
In order to establish a large-scale propagation and efficient genetic transformation system of Hydrangea macrophylla cv.Ivetta,the effects of different disinfection methods,basic medium types,hormone types and concentrations,transplanting substrate and other factors on adventitious bud induction,rooting and transplanting survival rate were studied by using Ivetta with bud shoots as explants.The results showed that Ivetta was disinfected by NaClO with an effective chlorine concentration of 2%for 10 min,and the contamination rate was low,and the optimal medium for axillary bud initiation was 1/2 B5+2.0 mg·L-1 6-BA;The optimal proliferation medium was B5+2.0 mg·L-1 6-BA;The optimal rooting medium was 1/2 B5+0.5 mg·L-1 IBA;The most suitable substrate for transplanting sterile seedlings was perlite∶peat soil=1∶1(V∶V).In this study,the tissue culture and rapid propagation technology system of Ivetta was preliminarily established,which could provide technical support for its factory seedling breeding and large-scale production.
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