大叶绣球艾薇塔组培快繁技术
Study on tissue culture and rapid propagation of Hydrangea macrophylla cv.Ivetta
陈双双 1秦紫艺 2邱帅 3冯景 1高凯 3齐香玉 1陈慧杰 1邓衍明2
作者信息
- 1. 江苏省农业科学院休闲农业研究所 林木资源高效生产全国重点实验室,江苏 南京 210014
- 2. 江苏省农业科学院休闲农业研究所 林木资源高效生产全国重点实验室,江苏 南京 210014;南京农业大学 园艺学院,江苏 南京 210008
- 3. 杭州市园林绿化股份有限公司,浙江 杭州 310009
- 折叠
摘要
为建立大叶绣球艾薇塔规模化繁殖和高效遗传转化体系,以艾薇塔带芽茎段为外植体,研究不同消毒方式、基本培养基类型、激素类型与浓度、移栽基质等因素对不定芽诱导、生根和移栽成活率的影响.结果表明,艾薇塔利用有效氯浓度为 2%的 NaClO 消毒 10 min,污染率较低;腋芽启动最适培养基为 1/2 B5+2.0 mg·L-1 6-苄基腺嘌呤(6-BA);最适增殖培养基为 B5+2.0 mg·L-1 6-BA;最佳生根培养基为 1/2 B5+0.5 mg·L-1 IBA;无菌苗最适移栽基质为珍珠岩∶泥炭土=1∶1(V∶V).研究初步建立了大叶绣球艾薇塔的组培快繁技术体系,可为其工厂化育苗和规模化生产提供技术支持.
Abstract
In order to establish a large-scale propagation and efficient genetic transformation system of Hydrangea macrophylla cv.Ivetta,the effects of different disinfection methods,basic medium types,hormone types and concentrations,transplanting substrate and other factors on adventitious bud induction,rooting and transplanting survival rate were studied by using Ivetta with bud shoots as explants.The results showed that Ivetta was disinfected by NaClO with an effective chlorine concentration of 2%for 10 min,and the contamination rate was low,and the optimal medium for axillary bud initiation was 1/2 B5+2.0 mg·L-1 6-BA;The optimal proliferation medium was B5+2.0 mg·L-1 6-BA;The optimal rooting medium was 1/2 B5+0.5 mg·L-1 IBA;The most suitable substrate for transplanting sterile seedlings was perlite∶peat soil=1∶1(V∶V).In this study,the tissue culture and rapid propagation technology system of Ivetta was preliminarily established,which could provide technical support for its factory seedling breeding and large-scale production.
关键词
大叶绣球/艾薇塔/组织培养/快速繁殖Key words
Hydrangea macrophylla/Ivetta/tissue culture/rapid propagation引用本文复制引用
基金项目
国家自然科学基金(32371956)
江苏省农业科技自主创新资金项目(CX222035)
江苏省种业振兴"揭榜挂帅"项目(JBGS[2021]097)
出版年
2024
NETL
NSTL
万方数据