Screening of culture medium for aseptic germination and strengthening cultivation of Hibiscus hamabo
Hibiscus hamabo is not only a preferred species for windbreak forests in southeast coastal areas,but also a good species for water conservation forests in barren mountains.The acquisition of aseptic seedlings is a prerequisite for the establishment of efficient regeneration systems,which could provide a basis for plant resource conservation and new variety selection and breeding.In this study,MS was used as the basic medium to compare the effects of additives such as citric acid,activated carbon,triiodobenzoicacid(TIBA),6-benzylaminopurine(6-BA),naphthalene acetic acid(NAA),casein hydrolyzed,vitamin H,folic acid,thidiazuron(TDZ),oligosaccharidic plant activator protein,etc.on the seed germination as well as the aseptic seedling cultivation of Hibiscus hamabo.The optimum formulation for seed germination was MS+6-BA(0.5 mg·L-1)+NAA(0.1 mg·L-1)+hydrolyzed casein(0.1 g·L-1),which increased the germination rate from 21.4%±7.8%to 85.3%±9.1%.Meanwhile,vitamin H,folic acid,and citric acid application failed to promote seed germination.Seedlings in medium of MS+6-BA(0.1 mg·L-1)+oligosaccharide chain protein(0.1 g·L-1)+activated carbon(0.1%)had the highest proliferation coefficient of 7.0±2.6 with thick stems,branches,and leaves.Notably,the combination of TDZ and NAA could induce the generation of Hibiscus hamabo with strong differentiation ability,which can provide a foundation for further developing the somatic embryo seedling technology of Hibiscus hamabo.
Hibiscus hamaboformulation selection6-benzylaminopurinehydrolyzed caseinoligosaccharide chain protein
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