Effect of TDZ on the proliferation and whitening effect of Spathiphyllum kochii
In order to establish an efficient and high-quality regeneration tissue culture system of Spathiphyllum kochii,the experiment was conducted to analyze the physiological and biochemical changes in the process of indefinite germination and explore the albino of its whitening effect.The single factor experimental design method was used to study the effect of thidiazuron(TDZ)on the adventitious bud proliferation and albino effect of the Spathiphyllum kochii.On the 5,10,15,20 and 25 d of culture,6 bottles were randomly selected to count the number of adventitious buds with effective proliferation(more than 0.5 cm)and effective rooting(more than 2.0 cm).Chlorophyll content,malondialdehyde content,proline content and CAT activity were determined.TDZ showed high inhibition and low promotion effects on the proliferation of adventitious buds of Spathiphyllum kochii,and the whitening rate of Spathiphyllum kochii gradually increased with the increase of TDZ concentration.The total whitening rate of Spathiphyllum kochii with 0.05 mg·L-1 TDZ treatment was significantly lower than that of other treatments.Proline content of Spathiphyllum kochii increased gradually with TDZ treatment time,MDA content of Spathiphyllum kochii increased first and then decreased,and CAT activity of Spathiphyllum kochii decreased with TDZ treatment time.
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